Elsevier

Journal of Chromatography A

Volume 822, Issue 1, 25 September 1998, Pages 117-123
Journal of Chromatography A

Regular paper
Determination of low-molecular-mass anionic compounds in beverage samples using capillary zone electrophoresis with simultaneous indirect ultraviolet and conductivity detection

https://doi.org/10.1016/S0021-9673(98)00563-9Get rights and content

Abstract

The determination of low-molecular-mass anionic components in beer samples using capillary zone electrophoresis with simultaneous direct conductivity and indirect UV detection is described. Optimization of the carrier electrolyte composition has been performed taking into account parameters such as pH as well as those that were found to be crucial for its compatibility with both detection principles employed in this paper. The latter are UV absorptivity, electrophoretic mobility of the buffer co-ion and the buffer co-ion concentration, which strongly influence the quality (signal-to-noise ratio, chromatographic resolution of adjacent peaks) of the electropherogram. Best results could be achieved with a carrier electrolyte consisting of 4-aminobenzoic acid, tetradecyltrimethylammonium bromide electroosmotic flow reversal and a pH of 5.75, adjusted using histidine. Using this carrier electrolyte, limits of detection ranging from 0.218 mg l−1 for pyroglutamate down to 0.018 mg l−1 for chloride could be obtained.

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    As a result, the multiplexing detection which could make up for the shortages of single detection and give more information about the analytes has received much attention. A variety of dual detection schemes have been proposed for capillary electrophoresis [33–45], while relatively less in connection with microfluidic chips [46–51]. Wang et al. [46] described the performance characteristics and advantages of a new dual electrochemical (EC) microchip detection system based on simultaneous conductivity and amperometric measurements.

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    Some carrier electrolytes have been reported as useful for the determination of low molecular weight organic acids in beverage samples. The majority of these carrier electrolytes were used for analysis of organic acids with indirect UV detection, as for example, benzoic acid [23], chromate [13,31], p-amino benzoic acid (PAB) [3,4,21], phthalate [14,20,32], 2,6-pyridinedicarboxylic acid (PDC) [6,10,15,16,22], pyromellitic acid (PMA) [18,19], and trimellitic acid (TMA) [9]. Regarding aspects such as the stability of the baseline, direct UV detection seems to be more suitable for the analysis of organic acids [33].

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