Elsevier

Journal of Chromatography A

Volume 510, 27 June 1990, Pages 3-11
Journal of Chromatography A

Review
Application of avidin—biotin technology to affinity-based separations

https://doi.org/10.1016/S0021-9673(01)93733-1Get rights and content

Abstract

During the last decade, avidin—biotin technology has become a commercially viable tool for research, medical and industrial applications. From the beginning, mediation via the avidin—biotin complex was proposed for affinity-based separations. This particular application, however, has been slow in gaining acceptance. One of the reasons is that the strength of binding between avidin and biotin is sometimes inapporpiate for the desired affinity system. Another problem involves certain “undesirable” structural properties in the avidin molecule which may lead to high levels of “non-specific” binding. Recent progress in understanding the molecular requirements for binding biotin may eventually lead to the design of avidin-like proteins which will exhibit preferred recognition properties according to teh desired application.

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