Rapid communicationOvercoming codon bias: A method for high-level overexpression of Plasmodium and other AT-rich parasite genes in Escherichia coli
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Acknowledgments
We thank James Kane, SmithKline Beecham Pharmaceuticals, for providing pRI952; Alan Cowman, Walter and Eliza Hall Institute for Medical Research (Melbourne, Australia) for providing the anti-DHPS antibody as well as pTrc-PPPK-DHPS from which the pET-DHPS expression system was derived; Ulrich Certa, Roche Pharmaceuticals, for providing the following Plasmodium aldolase expression vectors: p3217, p3255 and p3113; Victoria H. Brophy and Carol H. Sibley, Department of Genetics, University of
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2018, International Journal of Biological MacromoleculesCitation Excerpt :Another approach is the co-transformation of the host with a plasmid that codes for the rare tRNA’s. A number of reports confirm the concept of plasmid mediated tRNA complementation [83–85]. An example of such engineered strains is the E. coli Rosetta strain (Novagen, US) which carries tRNA genes for the rare codons AGG, AGA, AUA, CUA, CCC, and GGA.