The neurotoxic effect of 24-hydroxycholesterol on SH-SY5Y human neuroblastoma cells

doi:10.1016/S0006-8993(98)01274-8

Brain Research

Volume 818, Issue 1, 6 February 1999, Pages 171-175

https://doi.org/10.1016/S0006-8993(98)01274-8 Get rights and content

Abstract

Neurodegenerative disorders are characterized by a massive loss of nerve cells. The neuronal cell death is accompanied by an increased cholesterol release and conversion of cholesterol into the polar metabolite, 24-hydroxycholesterol (24-OH-Chol), appears to be an important mechanism in the central nervous system for eliminating cholesterol from the brain. We tested the influence of 24-OH-Chol on SH-SY5Y human neuroblastoma cells by recording cell morphology, Trypan blue exclusion, LDH-release into the culture medium, intracellular calcium and reactive oxygen species (ROS). The exposure of SH-SY5Y human neuroblastoma cells to 50 μM 24-OH-Chol led to a 90% loss in cell viability within 30 h, the LDH-release into the medium increased rapidly after 24 h, and after 24 to 30 h we found an elevation in intracellular calcium. These results show that, in a physiological concentration range, 24-OH-Chol damages neuronal cells, thus we speculate that this oxysterol may be involved in the etiology of neurodegenerative disease.

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Acknowledgements

We would like to gratefully acknowledge the late Professor Govind S. Rao who initiated this research project.

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Short communicationThe neurotoxic effect of 24-hydroxycholesterol on SH-SY5Y human neuroblastoma cells

Abstract

Section snippets

Acknowledgements

J. Lipid Res.

Neurochem. Int.

J. Biol. Chem.

FEBS Lett.

Neurosci. Lett.

Free Radic. Biol. Med.

Int. J. Biochem. Cell Biol.

Amyloid β-protein toxicity and oxidative stress in Alzheimer's disease

Cell Tissue Res.

Are reactive oxygen species involved in Alzheimer's disease?

Neurobiol. Aging

Neurotoxicity of 25-OH-cholesterol on NGF-differentiated PC12 cells

Neurochem. Res.

Short communication
The neurotoxic effect of 24-hydroxycholesterol on SH-SY5Y human neuroblastoma cells