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Brain Research
Volume 904, Issue 2, 22 June 2001, Pages 218-224
 
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doi:10.1016/S0006-8993(01)02461-1    
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Copyright © 2001 Elsevier Science B.V. All rights reserved.

Research report

The effect of feeding on cerebrospinal fluid corticotropin-releasing hormone levels in humans

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J. W. KasckowCorresponding Author Contact Information, E-mail The Corresponding Author, a, b, c, M. Haganb, J. J. Mulchaheya, b, D. G. Bakera, b, N. N. Ekhatora, b, J. R. Strawna, b, W. Nicholsond, D. N. Orthd, P. T. Loosene and T. D. Geracioti, Jr. a, b

a Cincinnati VAMC, Psychiatry Service, 3200 Vine St., Cincinnati, OH 45220, USA

b Department of Psychiatry, University of Cincinnati College of Medicine, 231 Bethesda Ave. (ML 559), Cincinnati, OH 45267-0559, USA

c University of Cincinnati Neurosciences Program, Cincinnati, OH 45267, USA

d Department of Internal Medicine, Division of Endocrinology, Vanderbilt University School of Medicine, Vanderbilt, USA

e Nashville VAMC and Department of Psychiatry, Vanderbilt University School of Medicine, Vanderbilt, USA


Accepted 27 March 2001. 
Available online 8 June 2001.

Abstract

Corticotropin-releasing hormone (CRH) is a neuropeptide thought to play a role in appetite regulation. In this report, we used a serial cerebrospinal fluid (CSF) sampling technique to examine the relationship between CSF CRH, plasma ACTH and cortisol and perceptions of hunger and satiety in fasting and sated volunteers. CSF was withdrawn continuously from 11:00 AM to 5:00 PM via an indwelling subarachnoid catheter. Blood was withdrawn every 10 min via an antecubital vein catheter. Fed subjects received a meal at 1:00 PM. Subjects who were fed had lower post-prandial ratings on hunger scales and higher ratings on satiety scales. Fed subjects also had slightly lower levels of CSF CRH after feeding. Furthermore, fed subjects had higher ACTH and cortisol concentrations in the first 3 h; by the fourth h the opposite was true. Our findings do not support the hypothesis that CNS CRH is a central satiety factor in the human. Instead our findings of slightly diminished CSF CRH levels after feeding may be accounted for by the rises in glucocorticoids and their associated negative feedback effects on CNS CRH. Alternatively, our findings could also reflect changes in CRH levels associated with feeding in multiple brain areas and in the spinal cord with the net effect being in the negative direction.

Author Keywords: Corticotropin-releasing hormone; Appetite; Satiety; Cerebrospinal fluid

Neuroscience classification codes: Neural basis of behavior, Neuropeptides and behavior

Article Outline

1. Introduction
2. Materials and methods
2.1. Continuous collection of cerebrospinal fluid
2.2. Visual analog scales
2.3. CRH, ACTH and cortisol radioimmunoassay
2.4. Statistical analysis
3. Results
3.1. Visual analog ratings
3.2. Neuroendocrine parameters
3.2.1. CRH
3.3. ACTH and Cortisol Levels
4. Discussion
Acknowledgements
References





Corresponding Author Contact Information Corresponding author. Tel.: +1-513-558-2989; fax: +1-513-558-4805; email: Jkasckow@pol.net


Brain Research
Volume 904, Issue 2, 22 June 2001, Pages 218-224
 
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