Biophysical Journal
Volume 74, Issue 5, May 1998, Pages 2223-2226
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Direct Observation of Ligand Colocalization on Individual Receptor Molecules

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Abstract

We have exploited the novel methodology of far-field fluorescence microscopy at the single molecule level to study colocalization of two different ligand molecules on an individual receptor. The use of dual-wavelength single molecule imaging allows discrimination between isolated and colocalized ligands with an accuracy of 40 nm. In the case of very close proximity of the two ligands, below 7 nm, single pair Förster energy-transfer was observed. The latter finding unequivocally demonstrates colocalization of two ligands on an individual receptor.

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