Original article: cardiovascularAutologous smooth muscle cell transplantation improved heart function in dilated cardiomyopathy
Section snippets
Experimental animals
The experimental animals used were male BIO 53.58 hamsters (BIO Breeders, Fitchburg, MA) weighing approximately 100 g. All procedures on these hamsters were approved by the Animal Care Committee of the Toronto General Hospital, and carried out in compliance with the “Guide to the Care and Use of Experimental Animals” of the Canadian Council on Animal Care and the “Guide for the Care and Use of Laboratory Animals” published by the National Institutes of Health (NIH publication 85-23, revised
Purity and labeling of cultured smooth muscle cells
The purity of the cultured smooth muscle cells, defined as positive immunohistochemical staining for α-smooth muscle actin, was 87.8% ± 3.4% (n = 6) just before transplantation (Fig 1). The efficiency of labeling with BrdU was 48.6% ± 6.5% (n = 2).
Histology
Severe focal myocardial necrosis and fibrosis were noted throughout the myocardium of the 21-week-old hamsters in all groups (Fig 2A). Four weeks after smooth muscle cell transplantation, a block of musclelike tissue was noted at the site of
Comment
Since the initial studies in which cultured AT-1 and fetal cardiomyocytes were transplanted into syngeneic mouse hearts 14, 15, significant progress has been achieved in the field of cell transplantation. Transplantation of cultured fetal and neonatal cardiomyocytes 5, 6, 7, skeletal muscle satellite cells 8, 9, and smooth muscle cells [10] has been shown to improve the function of scarred and infarcted hearts. Previously, we have evaluated the effect of allotransplantation of heart cells in
Acknowledgements
We thank Dev Olshansky and James C. Ho for the preparation and staining of the histologic materials and Drs Eung-Joong Kim and Shinji Tomita for surgical assistance. Ren-Ke Li is a Research Scholar of the Heart and Stroke Foundation of Canada. Richard D. Weisel is a Career Investigator of the Heart and Stroke Foundation of Ontario. This research was supported by Ren-Ke Li’s research grants from the Medical Research Council of Canada (MT-13665).
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