The human laminin β2 chain (S-Laminin): Structure, expression in fetal tissues and chromosomal assignment of the LAMB2 gene

doi:10.1016/0945-053X(95)90006-3

Matrix Biology

Volume 14, Issue 6, February 1995, Pages 489-497

https://doi.org/10.1016/0945-053X(95)90006-3 Get rights and content

Abstract

The sequence of the human laminin β2 chain (previously s-laminin) was derived from cloned cDNAs. The complete translation product has 1798 amino acid residues, including a 32-residue signal peptide. The human chain lacks the tripeptide sequence ILRE in domain I which is present in the rat polypeptide chain and has been shown to promote motor neuronal cell adhesion. The human gene (LAMB2 was localized to chromosome 3p21 using somatic cell hybrids and fluorescent in situ hybridization analysis. Northern and in situ hybridization analyses from numerous fetal tissues revealed that the β2 chain in generally widely expressed. β2, but not β1, was shown by in situ hybridization to be expressed in fetal brain and renal glomeruli. In fetal skin, β2 was expressed both in epidermal and dermal cells, while β1 was expressed only in the dermis. Expression of β2 in fetal liver was seen in hepatocytes, while no signals were observed for β1. In lung, both β1 and β2 were expressed in alveoli and bronchial smooth muscle cells, whereas only the β2 chain was expressed in bronchial epithelial cells. In striated muscle, however, the β1 chain, but not β2 was expressed. These results indicate different biological roles for the laminin β1 and β2 chains.

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Original paperThe human laminin β2 chain (S-Laminin): Structure, expression in fetal tissues and chromosomal assignment of the LAMB2 gene

Abstract

Matrix Biol.

J. Biol. Chem.

Dev. Biol.

Anal. Biochem.

J. Biol. Chem.

J. Biol. Chem.

Matrix

Cell

J. Biol. Chem.

Genomics

J. Biol. Chem.

J. Biol. Chem.

J. Biol. Chem.

J. Biol. Chem.

Matrix Biol.

Curr. Opin. Cell Biol.

Genomics

Herlitz's junctional epidermolysis bullosa is linked to mutations in the gene (LAMC2) for the γ2 subunit of nicein/kalinin (LAMININ-5)

Nat. Genet.

Synthesis of laminin and type IV collagen by trophoblastic cells and fibroblastic stromal cells in the early human placenta

Lab. Invest.

Structure and function of laminin: anatomy of a multidomain glykoprotein

FASEB J.

Cell adhesion, spreading and neurite stimulation by laminin fragment E8 depends on maintenance of secondary and tertiary structure in its rod and glubular domain

Eur. J. Biochem.

Merosin, a tissue-specific basemant membrane protein, is a laminin-like protein

Laminins and other strange proteins

Biochemistry

Distribution and isolation of four laminin variants; tissue restricted distribution of heterotrimers assembled from five different subunits

Cell Regul.

Isolation of human laminin B2 (LAMB2) cDNA clone and assignment of the gene to chromosome region 1q25–31

Cytogenet. Cell Genet.

Original paper
The human laminin β2 chain (S-Laminin): Structure, expression in fetal tissues and chromosomal assignment of the LAMB2 gene