Biochimica et Biophysica Acta (BBA) - Enzymology and Biological Oxidation
Short communicationRegulation of pyruvate dehydrogenase activity in Escherichia coli K12
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2022, Metabolic EngineeringCitation Excerpt :The experiments were performed by setting constant the total nucleotide pool at 2683 μM, value measured for the E. coli cytoplasm (Bennett et al., 2009), and varying the relative amounts of the oxidized and reduced forms. As shown in Fig. 7A, the Lpd R273H variant was several folds more efficient in the reductive reaction for a NAD+ mole fraction between 0.8 and 0.97, which is believed to correspond to the physiological range in aerobiosis (Hansen and Henning, 1966; Bennett et al., 2009; Shen and Atkinson, 1970). By contrast, the experiments showed no difference between the two enzymes in the oxidative reaction (Fig. 7B).
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2018, Biochimica et Biophysica Acta - BioenergeticsCitation Excerpt :Instead this surplus is often balanced by expending NADH in futile pathways or the synthesis of reduced by-products thereby lowering efficiency and yield [1]. Furthermore high cellular NADH concentrations can inhibit several central metabolic enzymes [3–5]. An example of a pathway with net NAD+ reduction is the conversion of glucose to organic acids such as citric, itaconic or malic acid.
Lactate fraction dependent mechanical properties of semitransparent poly(lactate-co-3-hydroxybutyrate)s produced by control of lactyl-CoA monomer fluxes in recombinant Escherichia coli
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Toward systematic metabolic engineering based on the analysis of metabolic regulation by the integration of different levels of information
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Permanent address: Biochemistry Department, Michigan State University, East Lansing, Mich., U.S.A.
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Present address: Max-PLank-Intitut für Biologie, Tübingen, Germany.