The incorporation of radioactive phosphate into the ribonucleic acid of cell-free preparations of Azotobacter vinelandii

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Abstract

  • 1.

    1. A cell-free preparation from Azotobacter vinelandii is capable of incorporating [32P]Pi into RNA in the presence of Mg2+ ions and 3-phosphoglyceric acid (or phosphoenol pyruvate).

  • 2.

    2. The 105 000 × g particulate fraction as well as the 105 000 × g supernatant fraction are essential for the incorporation.

  • 3.

    3. The incorporation is considerably stimulated by the addition of AMP, ADP, ATP or Poly-A at a concentration of 1·10−3 M. Higher concentrations usually lead to inhibition.

  • 4.

    4. The possibility of the labelling of RNA by the freely reversible reaction catalysed by polynucleotide phosphorylase has been ruled out.

  • 5.

    5. The radioactive RNA isolated from the incubation mixture behaves as s-RNA and all of its four nucleotidic components are radioactive.

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