Differential up-regulation of specific and azurophilic granule membrane markers in electropermeabilized neutrophils
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Alterations in blood leukocytes of G551D-bearing cystic fibrosis patients undergoing treatment with ivacaftor
2016, Journal of Cystic FibrosisCitation Excerpt :To examine the phenotype of leukocytes from CF patients with the G551D mutation, blood was stained for flow cytometric analysis before ivacaftor treatment and at one and six months post drug treatment. Included in the staining cocktails were markers of priming/activation including CD63 [a marker of degranulation [16]], the activation-specific epitope of CD11b [no cross-reactivity with native CD11b molecules on non-activated PMNs and monocytes [17]], and intracellular activity of the inflammasome-associated enzyme caspase-1 [18], which leads to the production of the potent pro-inflammatory cytokine interleukin-1β. The baseline levels of these markers before patients began treatment with ivacaftor were higher than levels found in age, sex, and race-matched healthy controls, and these levels decreased over the course of ivacaftor treatment.
A neutrophil intrinsic impairment affecting Rab27a and degranulation in cystic fibrosis is corrected by CFTR potentiator therapy
2014, BloodCitation Excerpt :This defect was also observed after stimulation with fMLF or fMLF in combination with TNF-α, indicating that the observed degranulation impairment was not stimuli specific (supplemental Figure 4). As an alternative approach, we investigated the membrane expression of CD66b.44 As illustrated in Figure 2F, up-regulation of CD66b to the plasma membrane was greatly decreased in CF neutrophils compared with HC cells (n = 8; P = .004, P = .01, and P = .004 after 5, 10, and 20 minutes, respectively).
Involvement of Munc18 isoforms in the regulation of granule exocytosis in neutrophils
2008, Biochimica et Biophysica Acta - Molecular Cell ResearchThe role of Rho GTPases and SNAREs in mediator release from granulocytes
2005, Pharmacology and TherapeuticsGTP-dependent secretion from neutrophils is regulated by Cdk5
2004, Journal of Biological ChemistryCitation Excerpt :This concurs with the inhibitory effect of roscovitine on the GTP-stimulated increase in surface expression of CD63 and CD66b in neutrophils. This increase in plasma membrane expression of CD63 and CD66b has been shown in the past to closely correlate with conventional assays of secretion from neutrophil azurophil and specific granules, respectively (30, 31). Although roscovitine may cause some inhibitory effect on the cell cycle Cdk1 and Cdk2, such effect may be disregarded in secretion by terminally differentiated neutrophils.
Transforming growth factor-β1 stimulates collagen matrix remodeling through increased adhesive and contractive potential by human renal fibroblasts
2004, Biochimica et Biophysica Acta - Molecular Cell Research