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doi:10.1016/0888-7543(92)90445-X 
Copyright © 1992 Published by Elsevier Inc.
The development and application of automated gridding for efficient screening of yeast and bacterial ordered libraries
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D. R. Bentleya, C. Todda, J. Collinsa, J. Hollanda, I. Dunhama, S. Hassocka, A. Bankierb and F. Giannellia
Received 1 July 1991;
Abstract
An automated gridding procedure for the inoculation of yeast and bacterial clones in high-density arrays has been developed. A 96-pin inoculating tool compatible with the standard microtiter plate format and an eight-position tablet have been designed to fit the Biomek 1000 programmable robotic workstation (Beckman Instruments). The system is used to inoculate six copies of 80 × 120-mm filters representing a total of 
20,000 individual clones in approximately 3 h. Highdensity arrays of yeast artificial chromosome (YAC) and cosmid clones have been used for rapid large-scale hybridization screens of ordered libraries. In addition, an improved PCR library screening strategy has been developed using strips cut from the high-density arrays to prepare row and column DNA pools for PCR analysis. This strategy eliminates the final hybridization step and allows identification of a single clone by PCR in 2 days. The development of automated gridding technology will have a significant impact on the establishment of fully versatile screening of ordered library resources for genomic studies.
