Construction of new β-glucuronidase cassettes for making transcriptional fusions and their use with new methods for allele replacement
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The role of solute binding proteins in signal transduction
2021, Computational and Structural Biotechnology JournalCitation Excerpt :A major conclusion of this study is that 87 % of the ligands modulated the expression of their cognate SBPs (Table 2). The magnitude of regulation stretches from a 3466-fold downregulation of phnD expression in the presence of 2 mM as compared to 0.2 mM Pi [87] to a 143-fold upregulation in the expression of tauA in the presence of taurine[88]. Although the regulatory proteins that control the expression of several of the SBPs have been identified [89–98], the corresponding molecular mechanisms of the regulation are not the focus of this review.
Labeled Azospirillum brasilense wild type and excretion-ammonium strains in association with barley roots
2017, Plant Physiology and BiochemistryCitation Excerpt :Both A. brasilense strains were used to construct mutants which carry a chromosomal PnifH-gusA fusion. The plasmid containing the PnifH-gusA fusion was constructed using the plasmids pSUP202::nifHDK of A. brasilense (Cbr Cmr Tcr; Souza, E. M.) and pWM6 (Metcalf and Wanner, 1993). The plasmid containing the structural genes of nitrogenase has two sites for the enzyme SacI inserted into the nifH gene and pWM6 plasmid releases the promoterless gusA-kanamycin (gusA-Km) cassette when treated with the same enzyme.
Gene expression of Pht cluster genes and a putative non-ribosomal peptide synthetase required for phaseolotoxin production is regulated by GacS/GacA in Pseudomonas syringae pv. phaseolicola
2011, Research in MicrobiologyCitation Excerpt :Chromosomal DNA from P. syringae strains was obtained as previously described (Chen and Kuo, 1993). The PCR-derived amplicon containing the gacA open reading frame was cloned into pUC19 (61) and interrupted at the NaeI site by introduction of the SmaI fragment from pWM6 containing uidA-aph genes (Metcalf and Wanner, 1993). Similarly, gacS was cloned in pCR4.0-TOPO (Invitrogen) and mutated by insertion of a PvuII fragment containing a tetracycline resistance cassette into a unique StuI site located within the gacS coding region.