Structurally stable Bacillus subtilis cloning vectors
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Production of recombinant human epidermal growth factor in Bacillus subtilis
2020, Journal of the Taiwan Institute of Chemical EngineersCitation Excerpt :Accordingly, B. subtilis is a suitable expression host for production of commercial and pharmaceutical enzymes, and is also one of the most frequently used bacteria for large-scale production [29,30]. To facilitate gene manipulation in B. subtilis, many studies have used E. coli-Bacillus shuttle vectors [31–34]. Plasmids used for the production of recombinant proteins in B. subtilis are known to be instable [35], because the plasmids currently used are rarely derived from Bacillus subtilis itself [36,37].
Promiscuous plasmid replication in thermophiles: Use of a novel hyperthermophilic replicon for genetic manipulation of Clostridium thermocellum at its optimum growth temperature
2016, Metabolic Engineering CommunicationsCitation Excerpt :Staphylococcus aureus plasmid pC194, that replicates by a rolling circle mechanism (Khan, 2005), as well as other Staphylococcus plasmids successfully transformed Bacillus subtilis (Dubnau, 1983; Ehrlich, 1977). Enterococcus faecalis plasmid pAMβ1 was used to transform Lactobacillus acidophilius (Luchansky et al., 1988) and B. subtilis (Jannière et al., 1990), and has since become a broad-range host vector for members of the Firmicutes. An example of interphyletic plasmid replication is pNG2, that was isolated from Corynebacterium diphtheriae, a member of the Actinobacteria phylum, but replicates in Escherichia coli, a member of the Proteobacteria phylum (Serwold-Davis et al., 1987).
Bacillus subtilis. Model Gram-Positive Synthetic Biology Chassis.
2013, Methods in MicrobiologyCitation Excerpt :A number of so-called theta-replicating plasmids have been developed as vectors for B. subtilis, including the enterococcal plasmid pAMβ1 (Bruand, Ehrlich, & Janniere, 1991; Janniere, Gruss, & Ehrlich, 1993) and pWV01 (Leenhouts & Venema, 1993) and the endogenous B. subtilis plasmids pLS20 (Koehler & Thorne, 1987; Meijer, de Boer, van Tongeren, Venema, & Bron, 1995) and pBS72 (Titok et al., 2003). Derivatives of the enterococcal plasmid pAMB1 have been used as the basis of a series of vectors for B. subtilis (Janniere, Bruand, & Ehrlich, 1990). For example, pHV1431, a B. subtilis/E. coli shuttle plasmid, carries the pBR322 replication functions for maintenance in E. coli and the pAMB1 replication functions for B. subtilis.
Medium optimization and production of secreted Renilla luciferase in Bacillus subtilis by fed-batch fermentation
2010, Biochemical Engineering JournalProduction of Recombinant Proteins in Bacillus subtilis
2007, Advances in Applied Microbiology