Construction and characterization of new cloning vehicles IV. Deletion derivatives of pBR322 and pBR325
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New high-cloning-efficiency vectors for complementation studies and recombinant protein overproduction in Escherichia coli and Salmonella enterica
2016, PlasmidCitation Excerpt :Addition of IPTG had the dual effect of relieving LacI repression and inducing transcription of genome-encoded T7 RNA polymerase. All vectors included a bla+ gene for the synthesis of β-lactamase, which provided resistance to ampicillin, an f1 origin for ssDNA packaging into phage capsids, and a pBR322 origin of replication (Bolivar, 1978; Soberon et al., 1980). The plasmid maps and MCSs of plasmids pTEV16-20 are shown in Fig. 4.
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