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doi:10.1016/0378-1119(89)90520-9 
Copyright © 1989 Published by Elsevier Science B.V.
Structure of 3'-downstream segment of the human smooth muscle (aortic-type) α-actin-encoding gene and isolation of the specific DNA probe
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Shinji Kamada
, Yoshiro Nakano and Takeo Kakunaga*
Received 10 March 1989;
Abstract
We isolated the 3'-downstream part of the human aortic smooth muscle α-actin (SMαA)-encoding gene and determined the nucleotide sequence, including the ninth (last) exon and 3'-untranslated (UT) region. From the comparison of the human 3'-UT region with rat and chicken 3'-UT regions, its homology is lower than those in 3'-UT regions of other actin isoforms such as cardiac a-actin and cytoskeletal β-actin. Therefore, by using the 3'-UT region of the human SMαA gene as an actin isoform-specific probe, this gene was detected as a single copy only in the human genome, which expressed the 1.7-kb RNA transcript in an aortic tissue-specific manner.
Author Keywords: Recombinant DNA; intron; exon; untranslated region; tissue-specific expression; Alu family; Z-DNA
Abbreviations: aa, amino acid(s); bp, base pair(s); kb, kilobase(s) or 1000 bp; nt, nucleotide(s); R, purine; SDS, sodium dodecyl sulfate; SMαA, smooth muscle α-actin; SMαA, gene (DNA) encoding SMαA; SSPE, 0.18 M NaCl/0.01 M NaH2PO4 pH 7.4/0.001 M EDTA; UT, untranslated; Y, pyrimidine
Article Outline
* Deceased: 21 September 1988.
