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doi:10.1016/0378-1119(89)90092-9 
Copyright © 1989 Published by Elsevier Science B.V.
Organization of the yeast URA2 gene: identification of a defective dihydroorotase-like domain in the multifunctional carbamoylphosphate synthetase-aspartate transcarbamylase complex
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J. L. Soucieta, 
, M. Nagyb, M. Le Gouarb, F. Lacroutec and S. Potiera
Received 25 November 1988;
Abstract
The 6636 bp of the yeast URA2 gene encoding the carbamoylphosphate synthetase-aspartate transcarbamylase complex have been sequenced. The protein is organized into four regions, three of which are functional domains as indicated previously by genetic analysis. The fourth domain corresponds to a defective dihydroorotase called DHOase-like. The URA2 gene complex with the same organization as the equivalent genes in higher eukaryotes suggests an evolution from a common ancestral gene.
Author Keywords: Recombinant DNA; Saccharomyces cerevisiae; pyrimidines; glutamine amido transferase; sequence comparisons; evolution
Abbreviations: aa, amino acid(s); ATCase (EC 2.1.3.2), aspartate transcarbamylase; a6U, 6-azauracil; bp, base pair(s); CAD, carbamoylphosphate synthetase-aspartate transcarbamylase-dihydroorotase; CPSase (EC 6.3.5.5), carbamoylphosphate syn-thetase; D.d., Dictyostelium discoideum; DHOase (EC 3.5.2.3), dihydroorotase; GAT, glutamine amide transfer; GATase, glutamine amido transferase; kb, 1000 bp; nt, nucleotide(s); oligo, oligodeoxyribonucleotide; ORF, open reading frame; pyrl-3-2, gene encoding the first three steps of the pyrimidine pathway in higher eukaryotes; SDS, sodium dodecyl sulfate; ss, single strand(ed); tsp, transcription start point(s); u, unit(s); UTP, uridine 5′ -triphosphate; wt, wild type; YNB, yeast nitrogen base medium
