Elsevier

Gene

Volume 74, Issue 2, 30 December 1988, Pages 503-515
Gene

Cloning and sequence determination of the gene encoding the largest subunit of the fission yeast Schizosaccharomyces pombe RNA polymerase I

https://doi.org/10.1016/0378-1119(88)90183-7Get rights and content

Abstract

The gene encoding the largest subunit of RNA polymerase I (SPRPA190) was cloned from the fission yeast Schizosaccharomyces pombe by cross-hybridization with a probe containing part of the corresponding Saccharomyces cerevisiae gene RPA190. The SPRPA190 gene is present in a single copy per haploid genome and is essential for cell growth. The polypeptide encoded by this gene, as deduced from the nucleotide sequence of the uninterrupted coding frame, consists of 1689 amino acids and its calculated Mr is 189300. The amino acid identity between the subunits of the two yeast species is 50%. Amino acid sequence conservation covers the regions previously suggested to be functionally important for the S. cerevisiae enzyme. In addition, two markedly hydrophilic regions recognized in the S. cerevisiae polypeptide can also be recognized in the S. pombe polypeptide in approximately the same positions, even though the amino acid sequences in these regions are diverged from each other. In the 5'-flanking region of the gene, several nucleotide sequence elements are detected which are also found in the two S. pombe ribosomal protein genes so far sequenced.

References (41)

  • M. Riva et al.

    Natural variation in yeast RNA polymerase A: formation of a mosaic RNA polymerase A in a meiotic segregant from interspecific hybrid

    J. Biol. Chem.

    (1982)
  • W.F. Schwindinger et al.

    Transcriptional elements of yeast ribosomal protein gene CYH2

    J. Biol. Chem.

    (1987)
  • M.L.M. Anderson et al.

    Quantitative filter hybridization

  • D. Beach et al.

    Construction of a Schizo-saccharomyces pombe gene bank in a yeast bacterial shuttle vector and its use to isolate genes by complementation

    Mol. Gen. Genet.

    (1982)
  • S. Broyles et al.

    Homology between RNA polymerases of poxviruses, prokaryotes, and eukaryotes: nucleotide sequence and transcriprional analysis of vaccinia virus genes encoding 147-kDa and 22-kDa subunits

  • M. Gribskov et al.

    Sigma factors from E. coliB. subtilis phage SP01, and phage T4 are homologous proteins

    Nucleic Acids Res.

    (1986)
  • T. Gross et al.

    Primary structure of the ribosomal protein gene S6 from Schizosaccharomyces pombe

    Curr. Genet.

    (1988)
  • J. Huet et al.

    A general upstream binding factor for genes of the yeast translational apparatus

    EMBO J.

    (1985)
  • H. Ito et al.

    Transformation of intact yeast cells treated with alkaline cations

    J. Bacteriol.

    (1983)
  • K. Kaiser et al.

    The use of phage lambda replacement vectors in the construction of representative genomic DNA libraries

  • Cited by (29)

    • Transcription in archaea

      1993, New Comprehensive Biochemistry
    View all citing articles on Scopus
    View full text