Progress in Neuro-Psychopharmacology
Radioimmunoassay of melatonin in rat serum
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Isolation of melatonin by immunoaffinity chromatography
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2002, General and Comparative EndocrinologyChronic administration of pharmacological levels of melatonin does not ameliorate the MPTP-induced degeneration of the nigrostriatal pathway
2001, Brain ResearchCitation Excerpt :Protein concentration was determined in an aliquot of each homogenate, and the concentrations of both DA and DOPAC were ultimately expressed as pmol per mg of protein. Thirty μl of each plasma sample were extracted into 750 μl of dichloromethane [12]. After centrifugation at 4°C, 600 μl of the organic phase were dried by vacuum aspiration, and the residue was reconstituted for radioimmunoassay (RIA) in 60 μl of RIA buffer (0.1% gelatin, 0.1 M Tricine, 0.9% sodium chloride, pH 8.0).
Circadian expression of clock genes in human oral mucosa and skin: Association with specific cell-cycle phases
2001, American Journal of PathologyCitation Excerpt :A second biopsy was taken from the oral mucosa from all study participants at the same times for a separate study of TS enzyme activity. Serum was separated from blood and stored at −70°C until assayed for cortisol (microparticle enzyme immunoassay, Abbot Diagnostics) and melatonin.25 Tissues were thawed and then minced using a homogenizer.
A novel and sensitive radioreceptor assay for serum melatonin levels
1991, Life SciencesInherited changes in concentrations of retinal and serum melatonin in the chicken
1989, General and Comparative Endocrinology