Elsevier

Neuroscience

Volume 60, Issue 1, May 1994, Pages 49-66
Neuroscience

Immunohistochemical localization in the rat brain of an epitope corresponding to the fibroblast growth factor receptor-1

https://doi.org/10.1016/0306-4522(94)90203-8Get rights and content

Abstract

The localization of fibroblast growth factor receptor-1 was investigated in rat brain by immunohistochemistry using a polyclonal antibody against an acidic peptide sequence of chicken fibroblast growth factor receptor-1. For raising the antisera in rabbits, we synthesized the oligopeptide EDDDDEDDSSSEEKEAD which is a highly acidic region of chicken fibroblast growth factor receptor-1. The oligopeptide was used as a haptenic antigen by conjugating with poly-l-glutamate as a carrier protein. On immunospot assay, the best antiserum was capable of detecting 15.7 pmols of both the chicken and its analogous human oligopeptides but failed to react even with up to 1 nmol of poly-l-glutamate. When rat brain homogenate was examined by Western blots, the antiserum revealed two bands with molecular weights of 145,000 and 75,000 corresponding to known sizes of the membrane-bound and secreted forms of the rat receptor, respectively. Immunohistochemistry in rat brain demonstrated that putative fibroblast growth factor receptor-1 immunoreactivity sites were present mainly in neurons but also in tanycytes and ependymal cells. Positive neurons were distributed widely in various brain regions, but were particularly abundant in such regions as the lateral hypothalamus, substantia nigra, locus coeruleus and raphe nuclei. The present study suggests that fibroblast growth factor receptor-1 is expressed preferentially in certain neuronal systems that appear to be under the influence of fibroblast growth factors in the normal brain. The result should facilitate study of the functional significance of fibroblast growth factors in these brain neurons.

References (54)

  • TooyamaI. et al.

    Production of antisera to acidic fibroblast growth factor and their application to immunohistochemical study in rat brain

    Neuroscience

    (1991)
  • TooyamaI. et al.

    High molecular weight basic fibroblast growth factor-like protein is localized to a subpopulation of mesencephalic dopaminergic neurons in the rat brain

    Brain Res.

    (1992)
  • UenoH. et al.

    A truncated form of fibroblast growth factor receptor 1 inhibits signal transduction by multiple types of fibroblast growth factor receptor

    J. biol. Chem.

    (1992)
  • WanakaA. et al.

    Localization of FGF receptor mRNA in the adult rat central nervous system byin situ hybridization

    Neuron

    (1990)
  • YanG. et al.

    Expression and transforming activity of a variant of the heparin-binding fibroblast growth factor receptor (flg) gene resulting from splicing of the alpha exon at an alternate 3′- acceptor site

    Biochem. biophys. Res. Commun.

    (1992)
  • AbeH. et al.

    Production of antiserum to [d-Ala2] deltorphin I and its immunohistochemical application to the mouse brain

    Neuroreport

    (1992)
  • BeanA.J. et al.

    Expression of acidic and basic fibroblast growth factors in the substantia of rat, monkey and human

  • BernardO. et al.

    Expression of two different forms of fibroblast growth factor receptor 1 in different mouse tissues and cell lines

  • BurgessW.H. et al.

    The heparin-binding (fibroblast) growth factor family of proteins

    A. Rev. Biochem.

    (1989)
  • CintraA. et al.

    Basic FGF is present in dopaminergic neurons of the ventral midbrain of the rat

    Neuroreport

    (1991)
  • DionneC.A. et al.

    Cloning and expression of two distinct high-affinity receptors cross-reacting with acidic and basic fibroblast growth factors

    Eur. molec. Biol. Org. J.

    (1990)
  • DuanD.S. et al.

    A naturally occurring secreted form of fibroblast growth factor (FGF) receptor 1 binds basic FGF in preference over acidic FGF

    J. biol. Chem.

    (1992)
  • EisemannA. et al.

    Alternative splicing generates at least five different isoforms of the human basic-FGF receptor

    Oncogene

    (1991)
  • EmotoN. et al.

    Basic fibroblast growth factor (FGF) in the central nervous system: identification of specific loci of basic FGF expression in the rat brain

    Growth Factors

    (1989)
  • FergusonI.A. et al.

    Fibroblast growth factor receptor-bearing neurons in the CNS: identification by receptor-mediated retrograde transport

    J. comp. Neurol.

    (1991)
  • FrautschyS.A. et al.

    Expression of basic fibroblast growth factor and its receptor in the rat subfornical organ

    Neuroendocrinology

    (1991)
  • GospodarowiczD.

    Localization of a fibroblast growth factor and its effect alone with hydrocortisone on 3T3 cell growth

    Nature

    (1974)
  • Cited by (69)

    • Endocrine mechanisms in obesity

      2018, Eating Disorders and Obesity in Children and Adolescents
    • Detection and Identification of Tissue Stem Cells: Tracking an Elusive Prey

      2010, Heart Development and Regeneration: Volume I
    • Fibroblast Growth Factor Receptor-1 is Required for Long-Term Potentiation, Memory Consolidation, and Neurogenesis

      2007, Biological Psychiatry
      Citation Excerpt :

      The FGF-2 is one of the major growth factors that stimulate the proliferation of embryonic neural stem cells in culture (Ghosh and Greenberg 1995; Vicario-Abejon et al. 1995). The FGF-2 and FGFR1 are expressed in the hippocampus (Gonzalez et al. 1995; Matsuo et al. 1994). To determine the role of FGF-2/FGFR1 signaling in adult neurogenesis in vivo, we have generated Fgfr1 conditional knockout (Fgfr1Co) mice with the Cre/loxP system (Xu et al. 2002).

    View all citing articles on Scopus
    View full text