Incorporation of the sulfur of L-[35S]methionine into the biotin molecule by intact cells of Rhodotorula glutinis

doi:10.1016/0304-4165(73)90237-7

Biochimica et Biophysica Acta (BBA) - General Subjects

Volume 304, Issue 3, 28 May 1973, Pages 887-890

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Abstract

The source of sulfur for biotin in microorganisms was studied. Using intact cells of Rhodotorula glutinis AKU 4847, L-methionine was much more effective for the synthesis of biotin from dethiobiotin than various other sulfur compounds tested. The reaction was carried out in the presence of L-[³⁵S]methionine. The radioactive biotin synthesized was isolated from the reaction mixture by a procedure involving cation- and anion-exchange column chromatographies, avidin treatment and membrane filtration, and then identified by radiochromatography and bioautography with Lactobacillus arabinosus. It was thus shown that the sulfur of methionine was incorporated into the biotin molecule by R. glutinis.

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BBA ReportIncorporation of the sulfur of L-[35S]methionine into the biotin molecule by intact cells of Rhodotorula glutinis

Abstract

J. Bacteriol.

J. Bacteriol.

J. Biol. Chem.

J. Bacteriol.

J. Bacteriol.

Agric. Biol. Chem.

Agric. Biol. Chem.

Biochim. Biophys. Acta

BBA Report
Incorporation of the sulfur of L-[³⁵S]methionine into the biotin molecule by intact cells of Rhodotorula glutinis