Molecular cloning and polymerase chain reaction-sequence-specific oligonucleotide detection of the allele encoding the novel allospecificity HLA-Cw6.2 (Cw∗1502) in Spanish gypsies

doi:10.1016/0198-8859(93)90509-Y

Human Immunology

Volume 37, Issue 4, August 1993, Pages 259-263

https://doi.org/10.1016/0198-8859(93)90509-Y Get rights and content

Abstract

A proposed novel allospecificity, HLA-Cw6.2, has been reported to be commonly found (∼25%) in Spanish Gypsies. Full-length cDNAs of the allele (Cw∗1502) coding for this antigen have been cloned in this study. A simple PCR-SSO method for its detection has been standardized and a correlation with the serologic Cw6.2 phenotype has been established. This specificity has been also detected in the homozygous typing cell RMI. Although the primary structures of Cw∗1502 and Cw∗0601 are not closely related, they share specific motifs that may account for their serologic cross-reactivity. Two novel HLA-C alleles (Cw∗12022 and Cw∗0602) are also reported.

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A new HLA-C allele, CW The name Cw 403 was officially assigned by the WHO Nomenclature Committee in July 1994. This follows the agreed policy that, subject to the conditions stated in the most recent Nomenclature Report (Bodmer et al. 1994), names will be assigned to new sequences as they are identified. Lists of such new names will be published in the following WHO Nomenclature Report. ...
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An allele encoding an HLA-C antigen, tentatively called CX44, associated with HLA-B44 was identified as a new member of the Cw l4 group, Cw^∗l403. The nucleotide sequence of Cw^∗l403 was closest to that of Cw^∗1401: five bases were different between the two alleles, in which three bases in Cw^∗l403 (two in exon 3 and one in exon 4) were the same as those of most HLA-C alleles. Two substitutions from guanine to adenine were found in the new allele, both of which are in exon 2, one at position 134 (61 of exon 2) and the other at position 201 (128 of exon 2). The former nucleotide substitution leads to the substitution of amino acid residue 21 from Arg to His, and the other substitution was synonymous. The former substitution was shared with Cw2, 3, 5, 13, and 15 alleles, and the latter was shared with Cw2, 4, 5, 8, 12, 13, 15, and 16 alleles. The other seven unrelated Japanese samples with CX44 were analyzed by a PCR-SSO method. It was confirmed that all the seven samples have the same substitutions as the sequenced allele, and the allele demonstrates a strong association with A33, B44, DR13, and DQ1, which are known to form a common haplotype in Japanese and Koreans.
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Cw^∗1602, a novel HLA-C allele belonging to the newly assigned Cw^∗16 group, has been cloned and sequenced from a Spanish Caucasoid cell expressing a “Cw6.2” phenotype. Some of the polymorphic substitutions of the new allele, and linkage disequilibrium to B51, had been predicted on the basis of previously published studies. The primary structure of Cw^∗1602 is in agreement with its serologic reactivity and, in comparison with that of Cw^∗1601, underlines the dimorphism of HLA-C molecules at residues 77 and 80 of the α₁-domain α helix.
Nomenclature for factors of the HLA system, 1994
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Molecular cloning and polymerase chain reaction-sequence-specific oligonucleotide detection of the allele encoding the novel allospecificity HLA-Cw6.2 (Cw∗1502) in Spanish gypsies

Abstract

Hum Immunol

Hum Immunol

Hum Immunol

Distribution of HLA antigens in Spanish Gypsies: a comparative study

Tissue Antigens

Analysis of class I and II antigens in Basque and Spanish Gypsy populations

Antigen Society no. 114: HLA-C

Microdroplet assay of human serum cytotoxicity

Nature

A DNA-RFLP typing system that positively identifies serologically well-defined and ill-defined HLA-DR and DQ alleles, including DRw 10

Transplantation

Description of the reference panel of B-lymphoblastoid cell lines for factors of the HLA system: the B-cell line panel designed for the Tenth International Histocompatibility Workshop