Differential chemotactic responses of different populations of fetal rat calvaria cells to platelet-derived growth factor and transforming growth factor β

https://doi.org/10.1016/0169-6009(92)90844-4Get rights and content

Summary

We tested the chemotactic response to platelet-derived growth factor (PDGF) and transforming growth factor β (TGFβ) of cells released enzymatically from fetal rat calvaria (RC). Both factors were chemotactic for RC cells, but the magnitude of the chemotactic response differed markedly between different populations and varied with time in culture of the cell populations. Cells released earlier from the calvaria showed a greater response than osteoblast-enriched populations released later. The optimal concentration of PDGF was the same for both alkaline phosphatase (AP)-positive and AP-negative cells within the populations. However AP-positive cells showed two peaks of response to TGFβ; one peak coincided with the TGFβ concentration also maximally affecting AP-negative cells, while the other occurred at a concentration 50–100 times higher. The results indicate that PDGF and TGFβ are chemotactic for both AP-positive and AP-negative cells in populations of cells derived from fetal calvariae, that chemotactic response declined with longer periods of time in culture, and that AP-positive osteoblast-like cells respond to a concentration of TGFβ that does not affect the AP-negative cells in the population.

References (28)

  • LandesmanR et al.

    Chemotaxis of muscle-derived mesenchymal cells to bone-inductive proteins of rat

    Calcif Tissue Int

    (1986)
  • MundyGR et al.

    Chemotactic activity of the γ-carboxyglutamic acid containing protein in bone

    Calcif Tissue Int

    (1983)
  • LucasPA et al.

    Chemotactic response of embryonic limb bud mesenchymal cells and muscle-derived fibroblasts to transforming growth factor β

    Connect Tissue Res

    (1988)
  • AubinJE et al.

    Isolation of bone cell clones with differences in growth,hormone responses, and extracellular matrix production

    J Cell Biol

    (1982)
  • Cited by (79)

    • Growth factors and cytokines in patients with long bone fractures and associated spinal cord injury

      2016, Journal of Orthopaedics
      Citation Excerpt :

      Recent researches in bone healing speculate the cause of these clinical observations to be due either to circulating humoral factors, which are released in the damaged brain or spinal cord, or through direct nerve signaling pathways. Neurohormonal or neurohumoral mechanisms were also suggested.16–30 Growth factors and cytokines are proteins that serve as signaling molecules for cells.

    • TGFβ-2 signaling is essential for osteoblast migration and differentiation during fracture healing in medaka fish

      2016, Bone
      Citation Excerpt :

      Among cytokines and growth factors in the healing process, transforming growth factor β (TGFβ) is one of the well-known factors that stimulate bone formation around bony tissues. The results of in-vitro studies revealed that TGFβ can act as a potent chemotactic factor to recruit osteoblasts to the site of bone formation [5]. TGFβ also stimulates both the proliferation and differentiation of pre-osteoblasts, but suppresses the later phase of osteoblast differentiation and mineralization [6].

    • Cellular and transcriptomic analysis of human mesenchymal stem cell response to plasma-activated hydroxyapatite coating

      2012, Acta Biomaterialia
      Citation Excerpt :

      In contrast to BMP, TGFB1 does not induce uncommitted MSC to differentiate along the osteogenic pathway [65]. Nonetheless, once these cells have become committed to the osteoblastic lineage TGFB1 can act as a potent chemotactic factor to recruit osteoblast precursors to sites of bone formation [66]. BMP4 acts synergistically with VEGF and significantly enhances bone formation and regeneration by improving bone healing through increasing MSC recruitment and survival [67].

    • The use of platelet-rich plasma in bone reconstruction therapy

      2009, Biomaterials
      Citation Excerpt :

      Controlled release of PDGF is beneficial to bone regeneration when applied in bone defects [68] or in periodontal defects [69], whereas high doses of PDGF may inhibit the bone regenerative process [70,71]. It appears that the primary effect of PDGF in bone regeneration is related to its mitogenic activity [72,73]. Serotonin (5-hydroxytryptamine, 5-HT) is not only a neurotransmitter but also a hormone with various extraneuronal functions [74].

    • Transforming Growth Factor-β

      2008, Principles of Bone Biology: Volume 1-2, Third Edition
    View all citing articles on Scopus
    View full text