Elsevier

Molecular Brain Research

Volume 1, Issue 3, December 1986, Pages 221-230
Molecular Brain Research

Research report
The metabolic turnover of the major proteins of the postsynaptic density

https://doi.org/10.1016/0169-328X(86)90028-8Get rights and content

Abstract

We have used the method of Austin, Lowry, Brown and Carter, to measure the steady-state metabolic half-life of tubulin (α and β individually) and actin (β and β together) in the total cytosolic (S3), microsomal (P3), synaptic plasma membrane (SPM) and synaptic junction (SJ) subcellular fractions from 6-day-old and adult chicken forebrain. In the SPM and SJ fractions we also measured the steady-state metabolic half-life of the major postsynaptic density protein (mPSDp). In SPM and SJ fractions from 6-day-old chickens tubulin and actin turned over approximately twice as slowly (t12 ≈ 24 days) as tubulin and actin in the S3 fraction (t12 ≈ 13 days). This difference was unlikely merely to be due to association with membranes since the t12 values for the proteins were the same in P3 and S3. The estimated t12 values for mPSDp were similar to that for tubulin and actin in SPM and SJ fractions. Similar results were obtained in adult chickens except that all t12 values in all fractions were approximately 30% larger. The calculated t12 values did not change between labelling periods of 4 and 6.5 h suggesting that the lag phase of incorporation of newly synthesized PSD proteins is sufficiently rapid to not produce this result artefactually. When the brain from a non-labelled chicken was homogenized in the presence of the S3 fraction from a labelled chicken and sub-fractionated the relative specific activities of the SPM and SJ fractions produced were 1–2% of those from the labelled brain. These results support the notion that tubulin and actin are intrinsic components of the PSD.

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    Present address: The Neuroscience Group, Faculty of Medicine, University of Newcastle, N.S.W. 2308, Australia.

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