Elsevier

Plant Science

Volume 79, Issue 2, 1991, Pages 253-257
Plant Science

Repression of plant tissue culture growth by light is caused by photochemical change in the culture medium

https://doi.org/10.1016/0168-9452(91)90114-NGet rights and content

Abstract

The effect of light on the growth of cultured Lycopersicon esculentum L., Daucus carota L. and Arabidopsis thaliana L. tissues was investigated. Callus growth from Arabidopsis leaf tissue was poor in the dark, but was stimulated by light filtered to remove UV and blue wavelengths. Tomato hypocotyl callus grew equally well in the dark and in filtered light. With respect to growth in the dark and filtered light, carrot hypocotyl callus was intermediate between the Arabidopsis and tomato tissues. However, for all three plant tissues, growth was reduced by unfiltered white light relative to the filtered light. The reduced growth caused by unfiltered white light can be attributed primarily to photochemical alterations of the culture medium rather than to photosensory functions of the plant tissues. Moreover, comparative studies with light-labile and light-stable auxins showed that the white-light-induced growth reduction was primarily due to photodegradation of components in the medium other than auxin.

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