Elsevier

Plant Science

Volume 61, Issue 2, 1989, Pages 227-234
Plant Science

Occurrence and partial characterization of an RNA species in rice mitochondria

https://doi.org/10.1016/0168-9452(89)90229-XGet rights and content

Abstract

An unusual species of RNA, designated RL-1, was detected in rice (Oryza sativa subsp. Japonica Li-ming A, CMS-Chinsurah Boro II type) mitochondria. Its molecular length was estimated to be 18 kb by using Bam HI fragments of Lambda DNA as markers. RL-1 RNA was not significantly affected by DNase I or by nuclease S1. It was not digested by RNase A under high salt conditions, but was completely digested under low salt conditions. When the total mitochondrial nucleic acids in 15% ethanol-saline-tris-EDTA (STE) solution were loaded on a microcrystalline cellulose chromatographic column, the mtDNA and low molecular RNAs were eluted by 15% ethanol-STE solution, but RL-1 molecules remained bound to the cellulose and were then eluted with STE buffer without ethanol. These results show that the RL-1 molecule may be double-stranded. Among the other ten rice cultivars surveyed, 18 kb RNA was detected in four all belonging to the same CMS-Chinsurah Boro II type (like Li-ming A), but not in five CMS-lines of rice of non-Chinsurah Boro II type or in fertile cultivar IR-24.

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