Antioxidant protection against organic and inorganic oxygen radicals by normal human plasma: the important primary role for iron-binding and iron-oxidising proteins

doi:10.1016/0167-4838(92)90052-F

Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology

Volume 1159, Issue 3, 20 October 1992, Pages 248-254

Biochimica et Biophy...

https://doi.org/10.1016/0167-4838(92)90052-F Get rights and content

Abstract

Normal human plasma contains numerous high- and low-molecular-mass redox-active molecules that are able to react rapidly with organic and inorganic oxygen radicals. The ability of such plasma molecules to substantially inhibit, or delay, free-radical mediated oxidation of added substrates has led to their classification as important biological antioxidants. Using phospholipids to detect organic oxygen radicals and deoxyribose to detect inorganic oxygen radicals, we here show that the primary antioxidants of normal human plasma reside mainly in two plasma proteins representing no more than 4% of the total proteins present. The iron-binding properties of transferrin and the iron-oxidising properties of caeruloplasmin, at a reaction dilution of 1:50, offer considerable protection against organic and inorganic oxygen radicals generated by iron and ascorbate. Plasma thiol-group-containing molecules, at concentrations well below those that would be required to complete with the detector molecule (based on known second-order rate constants for reaction with hydroxyl radicals) inhibited damage to deoxyribose, but stimulated damage to phospholipids.

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Antioxidant protection against organic and inorganic oxygen radicals by normal human plasma: the important primary role for iron-binding and iron-oxidising proteins

Abstract

Methods Enzymol.

J. Biol. Chem.

Chem. Biol. Interact.

FEBS Lett.

FEBS Lett.

Lancet

Anal. Biochem.

Anal. Biochem.

Biochim. Biophys. Acta

FEBS Lett.

FEBS Lett.

J. Free Rad. Biol. Med.

Clin. Chim. Acta

FEBS Lett.

Biochem. Biophys. Res. Commun.

Biochim. Biophys. Acta

FEBS Lett.

Biochim. Biophys. Acta

Biochim. Biophys. Acta

Chem. Phys. Lipids

Arch. Biochem. Biophys.

Int. J. Appl. Rad. Isotopes

Chem. Biol. Interact.

N. Engl. J. Med.

Annu. Rev. Pharmacol. Toxicol.