Anaphylatoxin C5a fails to promote prostacyclin release in cultured endothelial cells from human umbilical veins

doi:10.1016/0162-3109(86)90039-1

Immunopharmacology

Volume 12, Issue 2, October 1986, Pages 135-143

Immunopharmacology

https://doi.org/10.1016/0162-3109(86)90039-1 Get rights and content

Abstract

Subcultured endothelial cells from human umbilical veins respond to histamine and melittin with increased prostacyclin production, measured as 6-keto-prostaglandin F_1α by radioimmunoassay. However, no response to leukotriene C₄ was observed. Primary cultured cells, on the other hand, respond to leukotriene C₄ and the histamine response was 7 times more potent for these cells than for subcultured cells. In contrast, neither primary cultures nor subcultures of endothelial cells released prostacyclin following application of either human anaphylatoxin C5a (100 nM) or C3a (1 μM). In addition, these endothelial cells appear to have no specific binding sites for ¹²⁵I-C5a. However, endothelial cells released prostacyclin in the presence of human polymorphonuclear leukocytes that were activated with C5a. We conclude that involvement of endothelial cells in the haemodynamic response to anaphylatoxin is an indirect function, i.e. C5a activates circulating or tissue cells which in turn stimulate the endothelial cell to produce prostacyclin.

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^∗: Present address: Immunology, Centre Hospitalier de l'Université Laval, Quebec, Canada G1V 4G2.

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Anaphylatoxin C5a fails to promote prostacyclin release in cultured endothelial cells from human umbilical veins

Abstract

Prostaglandins

Cell

Biochem Biophys Res Commun

Thromb Res

Thromb Res

J Biol Chem

Thromb Res

Biochem Biophys Res Commun

Loss of receptor-mediated 86Rb efflux from pig aortic endothelial cells in culture

Br J Pharmacol

Interleukin-1 acts on cultured human vascular endothelium to increase the adhesion of polymorphonuclear leukocytes, monocytes and related leukocyte cell lines

J Clin Invest

Demonstration of specific C5a receptor on intact human polymorphonuclear leukocytes

Leukotriene C promotes prostacyclin synthesis by human endothelial cells

Uptake of 125I-labelled C3a by cultured human endothelial cells

Immunology

Modulating influence of chemotactic factor-induced cell adhesiveness on granulocyte function

J Clin Invest

Loss of receptor-mediated ⁸⁶Rb efflux from pig aortic endothelial cells in culture

Uptake of ¹²⁵I-labelled C3a by cultured human endothelial cells