Magnetically orientated tissue-equivalent tubes: application to a circumferentially orientated media-equivalent

doi:10.1016/0142-9612(96)85573-6

Biomaterials

Volume 17, Issue 3, 1996, Pages 349-357

https://doi.org/10.1016/0142-9612(96)85573-6 Get rights and content

Abstract

Circumferential orientation of collagen fibrils in a media-equivalent (ME) is achieved in a simple and effective way using the orientating effects of a strong magnetic field during collagen fibrillogenesis when the ME is first created. Circumferential orientation of the entrapped smooth muscle cells (SMC) is achieved subsequently via cell contact guidance, the induced SMC orientation along orientated fibrils. After describing the methods used, several lines of evidence are provided showing that the magnetically orientated ME is circumferentially orientated, including collagen birefringence, circumferential SMC orientation, accelerated ME compaction and increased ME stiffness with reduced creep in the circumferential direction as compared to control MEs not exposed to a magnetic field during fibrillogenesis. The optimization of these methods is discussed in order to better mimic the circumferential orientation and mechanical properties of a natural medium. Other applications of magnetically orientated tissue-equivalents are indicated.

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Biomaterials

Abstract

References (19)

In vitro construction of a human blood vessel from cultured vascular cells: a morphologic study

J Vasc Surg

New artificial connective matrix-like structure made of elastin solubilized peptides and collagens: elaboration, biochemical and structural properties

Biomaterials

Tissue engineering

Science

A blood vessel model constructed from collagen and cultured vascular cells

Science

Structural basis for the static mechanical properties of the aortic media

Circ Res

Mechanical stress induced cellular orientation and phenotypic modulation of 3-D cultured smooth muscle cells

ASAIO J

In vitro reconstruction of hybrid vascular tissue. Hierarchic and oriented cell layers

ASAIO J

Highly oriented, tubular hybrid vascular tissue for a low pressure circulatory system

ASAIO J

Cited by (158)

Biomaterials for Cardiovascular Tissue Engineering

Alignment of collagen matrices using magnetic nanowires and magnetic barcode readout using first order reversal curves (FORC) (invited)

Magnetic fields to align natural and synthetic fibers

6.14 Cardiovascular tissue engineering

Harnessing cellular-derived forces in self-assembled microtissues to control the synthesis and alignment of ECM

Characterization of sequential collagen-poly(ethylene glycol) diacrylate interpenetrating networks and initial assessment of their potential for vascular tissue engineering