Computer-assisted motility assessment of spermatozoa from fresh and frozen-thawed semen of the bull, boar and goat
References (9)
- et al.
Effect of different extenders on glutamic oxaloacetic transaminase (GOT) and glutamic pyruvic transaminase (GPT) release from frozen buffalo semen
Theriogenology
(1982) A new multiple exposure photography method for sperm motility determination
Fertil. Steril.
(1978)- et al.
Evaluation of six assays of sperm quality by means of their accuracy, precision and sensitivity in separating known induced levels of damage
- et al.
Experience with a computerized videomicrographic system for sperm analyses
Cited by (15)
Identification of sperm subpopulations with defined motility characteristics in ejaculates from Ile de France rams
2011, Animal Reproduction ScienceCitation Excerpt :The kinematic parameters recorded for each spermatozoon, as described by Mortimer (2000), were: curvilinear velocity, VCL (the average path velocity of the sperm head along its actual trajectory, μm/s); linear velocity, VSL (the average path velocity measured in a straight line from the beginning to the end of the track, μm/s); average path velocity, VAP (the average velocity of the smoothed cell path, μm/s); straightness, STR (the ratio between VSL and VAP, %); linearity, LIN (the ratio between VSL and VCL, %); wobble coefficient, WOB (the ratio between VAP and VCL, %); mean amplitude of lateral head displacement, ALH (the average value of the extreme side-to-side movement of the sperm head in each beat cycle, μm) and frequency of head displacement, BCF (frequency of the head crossing the average path trajectory, Hz). Spermatozoa were considered motile when VCL ≥ 20 μm/s (Farrell et al., 1998; Marco-Jiménez et al., 2005; Tuli et al., 1992), rapid (RAP) when VCL > 75 μm/s and progressive when STR was at least 80%. A multivariate cluster analysis was carried out in order to classify the spermatozoa into a reduced number of subpopulations according to their movement patterns so that every spermatozoon belonged to one and only one cluster.
Computer-Assisted Sperm Analysis for Assessing Initial Semen Quality and Changes during Storage at 5°C
1997, Journal of Dairy Science