Elsevier

Theriogenology

Volume 38, Issue 3, September 1992, Pages 487-490
Theriogenology

Computer-assisted motility assessment of spermatozoa from fresh and frozen-thawed semen of the bull, boar and goat

https://doi.org/10.1016/0093-691X(92)90068-3Get rights and content

Abstract

Generally, both subjective and computer-assisted (HTM-2000 motility analyzer) assessment of sperm motility in fresh and in frozen-thawed semen of bulls, boars and bucks yields comparable results. However, the use of a motility analyzer renders consistently more accurate estimates, especially when that motility is vigorous as in fresh bull semen.

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    The kinematic parameters recorded for each spermatozoon, as described by Mortimer (2000), were: curvilinear velocity, VCL (the average path velocity of the sperm head along its actual trajectory, μm/s); linear velocity, VSL (the average path velocity measured in a straight line from the beginning to the end of the track, μm/s); average path velocity, VAP (the average velocity of the smoothed cell path, μm/s); straightness, STR (the ratio between VSL and VAP, %); linearity, LIN (the ratio between VSL and VCL, %); wobble coefficient, WOB (the ratio between VAP and VCL, %); mean amplitude of lateral head displacement, ALH (the average value of the extreme side-to-side movement of the sperm head in each beat cycle, μm) and frequency of head displacement, BCF (frequency of the head crossing the average path trajectory, Hz). Spermatozoa were considered motile when VCL ≥ 20 μm/s (Farrell et al., 1998; Marco-Jiménez et al., 2005; Tuli et al., 1992), rapid (RAP) when VCL > 75 μm/s and progressive when STR was at least 80%. A multivariate cluster analysis was carried out in order to classify the spermatozoa into a reduced number of subpopulations according to their movement patterns so that every spermatozoon belonged to one and only one cluster.

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