A dextran swim-up procedure for separation of highly motile and viable ram spermatozoa from seminal plasma
References (34)
- et al.
Principles of cryopreservation and a review of stallion spermatozoa
J Equine Vet Sci
(1987) - et al.
Comparison of techniques for selection of motile spermatozoa
Fertil Steril
(1985) - et al.
The hypoosmotic swelling test: its employment as an assay to evaluate the functional integrity of the frozen-thawed bovine sperm membrane
Theriogenology
(1994) Effect of seminal plasma on the motility of epididymal and ejaculated spermatozoa of the ram and bull during the cryopreservation process
Theriogenology
(1994)- et al.
Clinical use of the turbidimetric analysis of sperm motility: comparison with visual techniques
Fertil Steril
(1981) - et al.
A quantitative method for determining the effects of drugs on spermatozoa motility
Fertil Steril
(1980) - et al.
A method for collecting motile spermatozoa from human semen
Fertil Steril
(1976) Rapid colorimetric assay of β-galactosidase and N-acetyl-β- glucosaminidase in human urine
Clin Chem Acta
(1976)- et al.
The effect of dialysis of extended ram semen prior to freezing on post-thaw survival and fertility
Cryobiology
(1986) - et al.
An increase in vitro fertilization ability of low-density human sperm capacitated by multiple-tube swim-up
Fertil Steril
(1987)
An assessment of sperm motility estimation for evaluation in rams
Theriogenology
Significance of reactive oxygen species and antioxidants in defining the efficacy of sperm preparation techniques
J Androl
Centrifugation of human spermatozoa induces sublethal damage; separation of human spermatozoa from seminal plasma by a dextran swim-up procedure without centrifugation extends their motile lifetime
Human Reprod
Survival of ram spermatozoa at high dilution: Protective effect of simple constituents of culture media as compared with seminal plasma
Reprod Fertil Dev
Effect of sperm motility on in vitro fertilization
Arch Androl
Poor hypo-osmotic swelling test results from cryopreserved sperm despite preservation of sperm motility
Arch Androl
Detrimental effects of cryopreservation on the structural and functional integrity of the sperm membrane
Arch Androl
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In vitro approach points to a chemotactic effect of melatonin on ram spermatozoa
2023, TheriogenologyInvolvement of progesterone and estrogen receptors in the ram sperm acrosome reaction
2021, Domestic Animal EndocrinologyUnderlying molecular mechanism in the modulation of the ram sperm acrosome reaction by progesterone and 17β-estradiol
2020, Animal Reproduction ScienceSephadex filtration as successful alternative to density-gradient centrifugation procedures for ram sperm selection with improved kinetics
2018, Animal Reproduction ScienceCitation Excerpt :The filtration, however, is believed to be based on the fact that non-viable sperm tend to adhere to the Sephadex matrix to a greater extent than motile and seemingly functional sperm, and the latter are able to cross the filtration barrier without modification of functional characteristics (Bussalleu et al., 2008). For ram semen, a number of sperm selection methods have been tried, including Sephadex filtration (see above), swim-up methods (García-López et al., 1996), sucrose washing, and Millipore filtration (Marti et al., 2006), as well as Percoll density gradient centrifugation (DGC) (Valcárcel et al., 1996). Even though there have been great advances in DGC procedures for sperm selection with different species (Santiago-Moreno et al., 2014, 2016), these procedures include centrifugal forces that may be harmful, especially for ram sperm (García-López et al., 1996).
Stallion sperm selection prior to freezing using a modified colloid swim-up procedure without centrifugation
2017, Animal Reproduction ScienceCitation Excerpt :However, the standard swim-up procedure involves centrifugation steps that induce a decline in the motility of spermatozoa (Alvarez et al., 1993). A swim-up method, without centrifugation, was developed by García-López et al. (1996) for the isolation of motile and highly viable ram spermatozoa. In order to optimize those results, an alternative procedure was developed by Dorado et al. (2016) for dog sperm selection before cooling using a column of colloid between the semen sample and the migration medium.