Cell
Volume 61, Issue 7, 29 June 1990, Pages 1349-1357
Journal home page for Cell

Article
ERD2, a yeast gene required for the receptor-mediated retrieval of luminal ER proteins from the secretory pathway

https://doi.org/10.1016/0092-8674(90)90698-EGet rights and content

Abstract

Resident proteins of the ER lumen carry a specific tetrapeptide signal (KDEL or HDEL) that prevents their secretion. We have previously described the isolation of yeast mutants that fail to retain such resident proteins within the cell. Here we describe ERD2, a gene required for retention. It encodes a 26 kd integral membrane protein whose abundance determines the efficiency and capacity of the retention system. Reduced expression of ERD2 leads to secretion of proteins bearing the HDEL signal, whereas overexpression of ERD2 improves retention both in wild-type cells and in other mutants. These results are consistent with other evidence that ERD2 encodes the HDEL receptor (see accompanying paper). The gene is also required, perhaps indirectly, for normal protein transport through the Golgi, and hence for growth. We discuss possible roles for ERD2 in the secretory pathway.

References (31)

  • T. Stevens et al.

    Early stages in the yeast secretory pathway are required for transport of carboxypeptidase Y to the vacuole

    Cell

    (1982)
  • J.D. Boeke et al.

    A positive selection for mutants lacking orotidine-5′-phosphate decarboxylase activity in yeast: 5-fluoro-orotic acid resistance

    Mol. Gen. Genet.

    (1984)
  • N. Dean et al.

    Recycling of proteins from the Golgi compartment to the ER in yeast

    J. Cell Biol.

    (1990)
  • G.I. Evan et al.

    Isolation of monoclonal antibodies specific for human c-myc proto-oncogene product

    Mol. Cell. Biol.

    (1985)
  • A. Franzusoff et al.

    Functional compartments of the yeast Golgi apparatus are defined by the sec7 mutation

    EMBO J.

    (1989)
  • Cited by (0)

    View full text