Cell
Volume 58, Issue 1, 14 July 1989, Pages 115-121
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Article
Expression of the v-erbA product, an altered nuclear hormone receptor, is sufficient to transform erythrocytic cells in vitro

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Abstract

We investigated the effect of the v-erbA oncogene product, an altered thyroid hormone receptor, in chicken erythrocyte progenitor cells. Bone marrow cells were infected with a retrovirus vector (XJ12) carrying the v-erbA gene in association with the neoR gene. XJ12-infected erythrocyte progenitor cells gave rise to G418-resistant clones. Some were composed of blast cells identified as transformed CFU-Es blocked in their differentiation. These cells could be grown in culture for at least 25 generations and required anemic chicken serum as a source of erythropoietic growth factors. XJ12 can infect erythrocyte progenitor cells in vivo but is not sufficient to induce erythroleukemia. These data suggest that the activation of a nuclear hormone receptor might represent one step toward the development of neoplasms.

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  • Cited by (0)

    Present address; Division of Biology, California Institute of Technology, Pasadena, California 91125.

    §

    On leave from the Tumor Virology Laboratory, Veteran's Administration Center and Department of Pathology, University of Florida Medical Center, Gainesville, Florida 32601.

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