Cell
Volume 35, Issue 1, November 1983, Pages 15-25
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Article
Generation of asymmetry and segregation of germ-line granules in early C. elegans embryos

https://doi.org/10.1016/0092-8674(83)90203-9Get rights and content

Abstract

Germ-line granules in C. elegans embryos (P granules) can be visualized by immunofluorescence microscopy using a monoclonal antibody. In mutant zygotes with abnormal spindle orientations and in wild-type zygotes treated with the microtubule inhibitors nocodazole, colcemid, vinblastine, and griseofulvin, both P-granule segregation to the posterior pole and the concomitant pseudocleavage occur apparently normally, but the normally concurrent migration of the pronuclei is inhibited. Conversely, treatment of wild-type embryos with the microfilament inhibitors cytochalasins D and B inhibits P-granule segregation and pseudocleavage, as well as other manifestations of polarity, without preventing pronuclear migration. The results suggest that P-granule segregation does not require either the spindle or cytoplasmic microtubules, but that this process as well as generation of other asymmetries does require cytoskeletal functions that depend on microfilaments.

References (42)

  • D. Taylor et al.

    Cytoplasmic structure and contractility in amoeboid cells

    Int. Rev. Cytol.

    (1979)
  • G. von Ehrenstein et al.

    Cell lineages and development of Caenorhabditis elegans and other nematodes

  • S. Ward et al.

    Fertilization and sperm competition in the nematode Caenorhabditis elegans

    Dev. Biol.

    (1979)
  • J. Wehland et al.

    Interaction of griseofulvin with microtubules, microtubule protein and tubulin

    J. Mol. Biol.

    (1977)
  • J.R. Whittaker

    Muscle lineage cytoplasm can change the developmental expression in epidermal lineage cells of ascidian embryos

    Dev. Biol.

    (1982)
  • W.B. Wood et al.

    Parental effects and phenotypic characterization of mutations that affect early development in Caenorhabditis elegans

    Dev. Biol.

    (1980)
  • D.G. Albertson

    Formation of first cleavage spindle in nematode embryos

    Dev. Biol.

    (1983)
  • M.C. Beckerle et al.

    Analysis of the role of microtubules and actin in erythrophore intracellular motility

    J. Cell Biol.

    (1983)
  • S. Brenner

    The genetics of Caenorhabditis elegans

    Genetics

    (1974)
  • D.R. Burgess et al.

    Polarized bundles of actin filaments within microvilli of fertilized sea urchin eggs

    J. Cell Biol.

    (1977)
  • E.M. Eddy

    Germ plasm and the differentiation of the germ cell line

    Int. Rev. Cytol.

    (1975)
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