Cell
ArticleHigher order structure of chromatin: Orientation of nucleosomes within the 30 nm chromatin solenoid is independent of species and spacer length
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Cited by (177)
Revisit of Reconstituted 30-nm Nucleosome Arrays Reveals an Ensemble of Dynamic Structures
2018, Journal of Molecular BiologyStructural insights of nucleosome and the 30-nm chromatin fiber
2016, Current Opinion in Structural BiologyStructure and organization of chromatin fiber in the nucleus
2015, FEBS LettersTopological polymorphism of the two-start chromatin fiber
2015, Biophysical JournalCitation Excerpt :The two-start fibers analyzed in these studies closely resemble the x-ray-based model by Schalch et al. (10) and belong to the family T2; the T1 conformations were not considered. According to the ED measurements (39–41), nucleosomes are strongly inclined in fibers. The angle between the nucleosome disks and the fiber axis, γ = | 90° – | ρ | |, is estimated to be ∼20° to 30° for various chromatin fibers with mixed internucleosome spacing.
Dynamics of modeled oligonucleosomes and the role of histone variant proteins in nucleosome organization
2013, Advances in Protein Chemistry and Structural BiologyCitation Excerpt :The crystal structure of a tetranucleosome evidences a two-start type of fiber (Schalch, Duda, Sargent, & Richmond, 2005). The solenoid fiber model adopts a hand-to-hand orientation by the consecutive nucleosomes, and the nucleosomal chains coil around an inner cavity with six to eight nucleosomes per turn with a pitch of ∼ 11 nm to form a one-start solenoid superhelix (Finch & Klug, 1976; McGhee, Nickol, Felsenfeld, & Rau, 1983; Thoma et al., 1979), in which nucleosome-stacking interactions occur between nearest neighbor nucleosomes (i + 1). To model this oligonucleosomes, a complete nucleosome structure with linker DNA is modeled using (i) the monomeric nucleosome structure (1F66/1EQZ) used for the global dynamics, (ii) a B-DNA of sequence 5′-CTGCAGATTCTACCAAAAG-3′ as the linker DNA region (after minimization) to facilitate the interaction with linker histone, and (iii) the structure of linker histone H1 from the Protein Data Bank (PDB): 1GHC (Cerf et al., 1994).
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Present address: Department of Medical Biochemistry, Faculty of Medicine, University of Calgary, Calgary, Alberta, Canada, T2N 1N4.