Cell
Characteristics of an SV40-plasmid recombinant and its movement into and out of the genome of a murine cell
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Papillomavirus DNA replication - From initiation to genomic instability
2009, VirologyCitation Excerpt :Elevated E1 protein levels facilitate too efficient initiation of replication compared to the elongation of the DNA synthesis. Such replication mode can be characterised by the “onion-skin”-type of replication (OSR) model, previously described by others (Botchan et al., 1979; Bullock et al., 1984; Hanahan et al., 1980; Syu and Fluck, 1997). This aberrant replication mode generates a complicated set of partially replicated fragments of the BPV1 genome, enriched with branching DNA strands, partially single stranded or double stranded, and abundant linear fragments, actively modified, recombined and degraded by the cellular machinery.
Stochastic gene expression in a lentiviral positive-feedback loop: HIV-1 Tat fluctuations drive phenotypic diversity
2005, CellCitation Excerpt :Subsequent experimental measurements of expression from the λ promoter and its operation in synthetic circuits confirmed its fundamentally stochastic nature (Elowitz and Leibler, 2000). There is also evidence that mammalian DNA viruses, such as simian virus 40 and cytomegalovirus, control lysogeny (or latency) life-cycle decisions without relying on PEV-like mechanisms (Hanahan et al., 1980; Reddehase et al., 2002). Here, we explore whether latency in the retrovirus HIV-1 can be explained by stochastic gene expression.
Transcriptionally active polymerase chain reaction (TAP). High throughput gene expression using genome sequence data
2002, Journal of Biological ChemistryCitation Excerpt :Several papers have recently appeared showing that PCR fragments containing promoter and terminator elements are transcriptionally active (1, 2, 9, 10). The observation that linear DNA fragments can be transcriptionally active was surprising to some investigators because the literature contains references reporting that supercoiling is required for efficient expression from plasmids (11-15). If supercoiling is indeed required for transcription, then one would not expect linear PCR fragments to be active.
Cat2 L-arginine transporter-deficient fibroblasts can sustain nitric oxide production
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Present address: Department of Zoology, Imperial College of Science and Technology, London, England.