Restoration of normal morphology, adhesion and cytoskeleton in transformed cells by addition of a transformation-sensitive surface protein

doi:10.1016/0092-8674(77)90322-1

Cell

Volume 11, Issue 1, May 1977, Pages 115-126

https://doi.org/10.1016/0092-8674(77)90322-1 Get rights and content

Abstract

Transformed cells lack a large, external, transformation-sensitive (LETS) glycoprotein which is a major surface component of their normal counter-parts. Addition of LETS glycoprotein isolated from normal cells to transformed cells restores certain morphological features and adhesive properties characteristic of normal cells. LETS protein is detected on the cell surface both by iodination using lactoperoxidase and by immunofluorescent staining. The surface distribution pattern detected by immunofluorescence is strikingly similar to that of normal cells. After addition of LETS protein, transformed cells also exhibit well defined actin cables which are not seen in untreated, transformed cells. All these alterations can be blocked by treating LETS protein with specific antisera or by subjecting it to mild trypsinization prior to addition to transformed cells. The effects are rapidly reversible by mild trypsinization, which removes the added LETS protein. The high rate of uptake of 2-deoxyglucose, characteristic of transformed cells, is not affected by LETS protein. These results suggest that LETS protein may have a role in cell attachment and spreading, and affect the organization of cytoskeleton.

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^∗: Present address: MRC Virology Unit, Institute of Virology, University of Glasgow, Church Street, Glasgow G11 5JR, Scotland.

^†: Present address: University of Pennsylvania, Philadelphia, Pennsylvania 19104.

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Cell

ArticleRestoration of normal morphology, adhesion and cytoskeleton in transformed cells by addition of a transformation-sensitive surface protein

Abstract

Exp. Cell Res.

J. Biol. Chem.

Exp. Cell Res.

Cell

Exp. Cell Res.

Biochim. Biophys. Acta

Exp. Cell Res.

Cell

Biochim. Biophys. Acta

J. Biol. Chem.

Biochim. Biophys. Acta

Adv. Cancer Res.

Virology

Cell

Virology

J. Biol. Chem.

Correlation between tumor induction and the large external transformation sensitive protein on the cell surface

Substrate-attached glycoproteins mediating adhesion of normal and virus-transformed mouse fibroblasts

J. Cell Biol.

Molecular composition and origin of substrate-attached material from normal and virus-transformed cells

J. Supramol. Structure

Substrate-attached serum and cell proteins in adhesion of mouse fibroblasts

J. Cell Physiol.

Transport changes rapidly inhibited by serum addition to “contact-inhibited” 3T3 cells

Immunofluorescence of mitotic spindles by using monospecific antibody against bovine brain tubulin

Science

Article
Restoration of normal morphology, adhesion and cytoskeleton in transformed cells by addition of a transformation-sensitive surface protein