Immunohistochemical study of low affinity Fc receptor for IgE in reactive and neoplastic follicles

doi:10.1016/0090-1229(89)90059-7

Clinical Immunology and Immunopathology

Volume 53, Issue 2, November 1989, Pages 309-320

Clinical Immunology ...

https://doi.org/10.1016/0090-1229(89)90059-7 Get rights and content

Abstract

The distribution of low affinity IgE Fc receptors (FcϵR₂, FceRII) in reactive and neoplastic follicles was studied by an indirect immunoperoxidase method with monoclonal antibody specifically reacting to FceRII (H107). The tissues examined in this study included lymph nodes, extranodal tissues of divergent diseases, and follicular lymphomas. In the germinal centers (GCs) of the lymph follicles, FceRII showed a lace-like pattern irrespective of the distribution of IgE. In general, FceRII was positive only in the light zone and not in the dark zone of GCs. The distribution of FceRII was different from that of DRC1 which was always present in the entire portion of GC. There were two types of DRC1(+) FDC which were FceRII(+) and FceRII(−). FceRII was seen by immunoelectron microscopy on the cell surface of follicular dendritic cells (FDCs). IgE-positive GCs in Kimura's disease and Warthin's tumor were intensely positive for FceRII in their entire portion. In IgE-positive GCs, an increased number of FceRII-positive lymphoid GC cells was recognized by immunoelectron microscopic observation. In follicular lymphoma, there were also two types of FDC which were FceRII(+) and FceRII(−). These findings indicated that FceRII on FDCs was closely related to the IgE immune response and also was a marker for functional phase or differentiation of FDCs.

References (40)

H.L. Spiegelberg
Structure and function of Fc receptors for IgE on lymphocyte, monocyte and macrophage
Adv. Immunol.
(1984)
A.G. Farr et al.
Immunohistochemistry with enzyme labeled antibodies: A brief review
J. Immunol. Methods
(1981)
T. Nagai et al.
T and B lymphocyte with immunoglobulin E Fc receptor (FcϵR) in patients with nonallergic hyperimmunoglobulinemia E: Demonstration using a monoclonal antibody against FcϵR-associated antigen
Clin. Immunol. Immunopathol.
(1985)
T. Kasajima et al.
Immunohistochemical study of intrathyroidal lymph follicle
Clin. Immunol. Immunopathol.
(1987)
S. Kanowith-Klein et al.
Expression of Fc receptors and surface and cytoplasmic IgE on human fetal and adult lymphopoietic tissue
Clin. Immunol. Immunopathol.
(1988)
H. Stein et al.
The normal and malignant germinal center
Clin. Hematol.
(1982)
E. Ishikawa et al.
Pathologic study on eosinophilic lymphfolliculoid granuloma (Kimura's disease)
Acta. Pathol. Japan.
(1981)
T. Kasajima et al.
Patho-morphology of the germinal center of lymph follicle in Kimura's disease
J. Japan. Soc. Reticuloendothel. Syst.
(1986)
A. Masuda
Immunohistochemical study of Warthin's tumor, especially of the germinal center
Histol. Histopathol.
(1988)
T. Tada et al.
Distribution of γE-forming cells in lymphoid tissue of the human and monkeys
J. Immunol.
(1970)

K.J. Gajl-Peczalska et al.

Localization of immunological complexes fixing β1c (C3) in germinal center of lymph node

J. Exp. Med.

(1969)

N. Van Rooijin

Immune complex in the spleen: Three concentric areas of immune complex trapping, their interrelationships and possible function

J. Reticuloendothel. Soc.

(1977)

G.G.B. Klaus et al.

The follicular dendritic cell: Its role in antigen presentation in the generation of immunological memory

Immunol. Rev.

(1980)

J. Gerdes et al.

Complement (C3) receptors on dendritic reticulum cells of normal and malignant lymphoid tissue

Clin. Exp. Immunol.

(1982)

J.G. Tew et al.

The maintenance and regulation of the humoral immune response: Persisting antigen and the role of follicular antigen-binding dendritic cells as accessory cells

Immunol. Rev.

(1980)

Y. Imai et al.

Function of follicular dendritic cell in the germinal center of lymphoid follicle

Histol. Histopathol.

(1986)

M. Suemura et al.

Monoclonal anti-Fc_ϵ receptor antibodies with different specificities and studies on the expression of Fc receptors on human B and T cells

J. Immunol.

(1987)

J. Yodoi et al.

Induction of Fc_ϵ receptor bearing cells in vitro in human peripheral lymphocyte

J. Immunol.

(1980)

A. Gonzalez-Molina et al.

A subpopulation of normal human peripheral B lymphocytes that bind IgE

J. Clin. Invest.

(1977)

F.M. Melewicz et al.

Comparison of the Fc receptor for IgE on human lymphocyte and monocyte

J. Immunol.

(1982)

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Immunohistochemical study of low affinity Fc receptor for IgE in reactive and neoplastic follicles

Abstract

Adv. Immunol.

J. Immunol. Methods

Clin. Immunol. Immunopathol.

Clin. Immunol. Immunopathol.

Clin. Immunol. Immunopathol.

Clin. Hematol.

Pathologic study on eosinophilic lymphfolliculoid granuloma (Kimura's disease)

Acta. Pathol. Japan.

Patho-morphology of the germinal center of lymph follicle in Kimura's disease

J. Japan. Soc. Reticuloendothel. Syst.

Immunohistochemical study of Warthin's tumor, especially of the germinal center

Histol. Histopathol.

Distribution of γE-forming cells in lymphoid tissue of the human and monkeys

J. Immunol.

Localization of immunological complexes fixing β1c (C3) in germinal center of lymph node

J. Exp. Med.

Immune complex in the spleen: Three concentric areas of immune complex trapping, their interrelationships and possible function

J. Reticuloendothel. Soc.

The follicular dendritic cell: Its role in antigen presentation in the generation of immunological memory

Immunol. Rev.

Complement (C3) receptors on dendritic reticulum cells of normal and malignant lymphoid tissue

Clin. Exp. Immunol.

The maintenance and regulation of the humoral immune response: Persisting antigen and the role of follicular antigen-binding dendritic cells as accessory cells

Immunol. Rev.

Function of follicular dendritic cell in the germinal center of lymphoid follicle

Histol. Histopathol.

Monoclonal anti-Fcϵ receptor antibodies with different specificities and studies on the expression of Fc receptors on human B and T cells

J. Immunol.

Induction of Fcϵ receptor bearing cells in vitro in human peripheral lymphocyte

J. Immunol.

A subpopulation of normal human peripheral B lymphocytes that bind IgE

J. Clin. Invest.

Comparison of the Fc receptor for IgE on human lymphocyte and monocyte

J. Immunol.

Monoclonal anti-Fc_ϵ receptor antibodies with different specificities and studies on the expression of Fc receptors on human B and T cells

Induction of Fc_ϵ receptor bearing cells in vitro in human peripheral lymphocyte