Specialized transduction of tetracycline resistance by phage P22 in Salmonella typhimurium: II. Properties of a high-frequency-transducing lysate
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Reactive Enamines and Imines In Vivo: Lessons from the RidA Paradigm
2019, Trends in Biochemical SciencesCitation Excerpt :In some cases, the cofactor-bound enamine is protonated to an iminium ion prior to release from the enzyme (Figure 1B) [31,32]. One example includes tryptophanase (TnaA; EC 4.1.99.1), a versatile fold-type I PLP-dependent lyase responsible for tryptophan catabolism (via α,β-elimination) [33], cysteine detoxification (α,β-elimination) [34], and tryptophan synthesis (β-substitution) [35]. Iminium ions are also produced as catalytic intermediates by FAD-dependent dehydrogenases (Figure 1C).
tRNA Methylation Is a Global Determinant of Bacterial Multi-drug Resistance
2019, Cell SystemsCitation Excerpt :The mutation was confirmed via sequencing (data not shown). A clone containing the mutation but without phage contamination was isolated using a green plate (Chan et al., 1972) and the mutated locus was transferred to Salmonella trmD-KD with the maintenance plasmid expressing human trm5. The desired clone was selected with the kanR marker, which was removed via pCP20-mediated FLP recombination to leave a scar.
Analyses of variants of the Ser/Thr dehydratase IlvA provide insight into 2-aminoacrylate metabolism in Salmonella enterica
2018, Journal of Biological ChemistryUnderstanding the multifaceted roles of the phosphoenolpyruvate: Phosphotransferase system in regulation of Salmonella virulence using a mutant defective in ptsI and crr expression
2019, Microbiological ResearchCitation Excerpt :For construction of the transcriptional lacZY fusion, the resolved strain with a recombination scar in place of the kan resistance cassette was transformed with pCE70 to replace the scar sequence with lacZY (Ellermeier et al., 2002). In the case of horizontal transfer of genetic materials between Salmonella strains, including SL1344, 14028, and UK1 strains, the phage P22-mediated transduction was used as described previously (Chan et al., 1972). To construct a plasmid pPC, pts1/pts2 and pts3/pts4 primer sets were used to amplify the promoter region for the ptsHIcrr operon (447 bp) and ptsI and crr DNA fragments (2275 bp).