Racemization studies of Fmoc-Cys(Trt)-OH during stepwise Fmoc-Solid phase peptide synthesis
During conventional stepwise Fmoc-based solid phase peptide synthesis, base-catalyzed acylation methods lead to considerable racemization of Cys(Trt)- assembled peptide fragments during the activation/coupling process. As demonstrated in this communication, the epimerization of Fmoc-Cys(Trt)-OH can be reduced to a negligible degree, applying an activation step in a neutral medium.
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