Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis
Research paperSuppressors of Escherichia coli mutT: antimutators for DNA replication errors
References (17)
General antimutators are improbable
J. Mol. Biol.
(1993)- et al.
The Escherichia coli galK2 papillation assay: its specificity and application to seven newly isolated mutator strains
Mutation Res.
(1993) - et al.
Motif A of bacteriophage T4 DNA polymerase: role in primer extension and DNA replication fidelity. Isolation of new antimutator and mutator DNA polymerases
J. Biol. Chem.
(1994) Base selection, proofreading, and mismatch repair during DNA replication in Escherichia coli
J. Biol. Chem.
(1993)- et al.
A single mutation in bacteriophage T4 DNA polymerase (A737V, tsL141) decreases its processivity as a polymerase and increases its processivity as a 3′ → 5′ exonuclease
J. Biol. Chem.
(1994) Linkage map of Escherichia coli K-12
A constant rate of spontaneous mutation in DNA-based microbes
- et al.
Mutants of Escherichia coli with increased fidelity of DNA replication
Genetics
(1993)
Cited by (22)
Replication fidelity in E. coli: Differential leading and lagging strand effects for dnaE antimutator alleles
2019, DNA RepairCitation Excerpt :In the present work, we have analyzed in detail the effects of the three dnaE alleles (dnaE911, dnaE915, and dnaE941) that exhibit strong antimutator phenotypes in a mismatch- repair-defective background, and hence are characterized by significantly lowered error rates [13–15]. They each carry a mutation in the alpha subunit of DNA Pol III [15,16]. We investigated (i) the strength of the antimutator effects, (ii) the strand dependence of these effects, and (iii) their behavior in the presence of one of several error-prone polymerases.
Fluorescence-Based Reporters for Detection of Mutagenesis in E. coli
2017, Methods in EnzymologyCitation Excerpt :Spotting the surface of these colonies, each colored papilla (sectors) represents a microcolony derived from a single Gal + mutant capable of galactose fermentation. The output is semiquantitative, though, as it depends on mutation events occurring early enough to allow for visual detection (Schaaper, 1996). Here, we present two sets of reporters for in vivo mutagenesis that produce a quantitative output, and thus have the potential to greatly reduce the amount of test chemical and labor involved in these assays.
Sequence analysis and phenotypes of five temperature sensitive mutator alleles of dnaE, encoding modified α-catalytic subunits of Escherichia coli DNA polymerase III holoenzyme
2002, Mutation Research - Fundamental and Molecular Mechanisms of MutagenesisThe antimutator phenotype of E. coli mud is only apparent and results from delayed appearance of mutants
2001, Mutation Research - Fundamental and Molecular Mechanisms of MutagenesisEffect of host bacteria genotype on spontaneous reversions of Bacillus subtilis bacteriophage Φ29 sus17 nonsense codon
2000, FEMS Microbiology LettersEffect of Escherichia coli dnaE antimutator mutants on mutagenesis by the base analog N<sup>4</sup>-aminocytidine
1998, Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis