Elsevier

Life Sciences

Volume 57, Issue 9, 21 July 1995, Pages 873-879
Life Sciences

Chromatographic evidence for amadori product formation in rat liver aminophospholipids

https://doi.org/10.1016/0024-3205(95)02020-JGet rights and content

Abstract

This work presents data which indicates the presence of Amadori product derived from Maillard reaction in aminophospholipids. The presence of 5-HMF, a stable derivative of acid-treated Amadori product, in the phospholipidic fraction from cell membranes was established by HPLC-UV and subsequent GC/MS analysis. The assay for 5-HMF in rat liver phospholipid revealed the presence of this molecule in membrane phospholipids of euglycemic rats, and showed increased glycation levels in membrane phospholipids from streptozotocin-induced diabetic rats (p < 0.001). This gives a new insight to cell membrane physiology and physiopathology.

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