Elsevier

Life Sciences

Volume 27, Issue 22, 1 December 1980, Pages 2063-2069
Life Sciences

Isolation of selective 3H-chlornaltrexamine-bound complexes, possible opioid receptor components in brains of mice

https://doi.org/10.1016/0024-3205(80)90485-3Get rights and content

Abstract

The nonequilibrium narcotic antagonist, chlornaltrexamine (CNA) was used to bind selectively and covalently pioid specific sites on brain membrane preparations. Selective binding of [3H]CNA occured with a saturation maximum of 185 fmol/mg protein. Bound [3H]CNA was extracted with Triton X-100, dialyzed against Brij 36T, precipitated with trichloroacetic acid and chromatographed on an ultrogel AcA 22 column. The elution profile suggests that this extract contains a minimum of four selective [3H]CNA complexes. At least two of these complexes migrate in a single large peak. Column calibration showed that this peak eluted at 590,000 daltons. One of these specific [3H]CNA complexes elutes at the elution volume of the column and is dialyzable. Finally, putative aggregate of these complexes elutes with the void volume.

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Supported by U.S. Public Health Service Grants DA 00289 and DA 01533.

2

Supported in part by U.S. Public Health Service Grant GM 07397.

3

Department of Medicinal Chemistry, School of Pharmacy.

4

Department of Pharmacology, Medical School.

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