Proctolinergic innervation of the accessory gland in male crickets (Gryllus bimaculatus): Detection of proctolin and some pharmacological properties of myogenically and neurogenically evoked contractions

doi:10.1016/0022-1910(89)90011-5

Journal of Insect Physiology

Volume 35, Issue 3, 1989, Pages 251-257, 259-264

https://doi.org/10.1016/0022-1910(89)90011-5 Get rights and content

Abstract

Spontaneous contractions were observed in the cricket male accessory gland which is innervated by dorsal unpaired median neurones (DUMR7 neurones) and paired neurones (LC neurones) arising from the terminal abdominal ganglion. These contractions were myogenic: identical mechanical activity was observable in a transplanted accessory gland which lacked innervation. Selective antidromic electrical stimulation of the DUMR7 neurones evoked a contraction of the accessory gland, which was graded and dependent upon both frequency and duration of stimulation. Glutamate at 10⁻⁴ M, and proctolin, at low concentrations of 10⁻⁹ M, produced sustained contractions of the accessory gland. These drugs at higher concentrations (glutamate, > 10⁻⁴ M; proctolin, > 10⁻⁸ M) reduced the amplitude of neurogenically evoked contractions and increased the basal tonus. Octopamine, at 10⁻⁷ M, increased the frequency of myogenically evoked contractions. Application of serotonin, at 10⁻⁵ M, reduced the amplitude of neurally evoked contractions and also resulted in a lowering of the basal tonus. By use of high-performance liquid chromatoraphy and bioassay we have found that proctolin was present in the accessory gland, and it was released from the accessory gland in response to the application of high K⁺ saline. No proctolin was detected in the transplanted accessory gland.

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Proctolinergic innervation of the accessory gland in male crickets (Gryllus bimaculatus): Detection of proctolin and some pharmacological properties of myogenically and neurogenically evoked contractions

Abstract

Life Sci.

J. Insect Physiol.

J. Insect Physiol.

Neuroscience

J. Insect Physiol.

Comp. Biochem. Physiol.

Adv. Insect physiol.

Comp. Biochem. Physiol.

Brain Res.

J. Insect Physiol.

J. Insect Physiol.

The distribution of a proctolin-like immunoreactive material in the terminal ganglion of the cockroach, Periplaneta americana L.

Cell Tiss. Res.

Identification of the neuropeptide transmitter proctolin in Drosophila larvae: characterization of muscle fiber-specific neuromuscular endings

J. Neurosci.

Postembryonic development of insect motor systems

Neuropeptide proctolin (H-Arg-Tyr-Leu-Pro-Thr-OH): Immunocytochemical mappings of neurons in the central nervous system of the cockroach

J. comp. Neurol.

Neuropeptide proctolin (H-Arg-Tyr-Leu-Pro-Thr-OH): Immunological detection and neuronal localization in insect central nervous system

Neuromuscular transmitter substance in insect visceral muscle

Science

Metamorphosis of flight motor neurones in the moth Manduca sexta

J. comp. Physiol.

Symmetrically organized dorsal unpaired median (DUM) neurones and flash control in the male firefly, Photuris versicolor

J. exp. Biol.

The neurophysiology of larval firefly luminescence: direct activation through four bifurcating (DUM) neurons

J. comp. Physiol.

Structural and functional changes in an identified cricket neuron after separation from the soma. I: Structural changes

J. comp. Neurol.

The action of proctolin and 5-hydroxytryptamine on the oviduct of the horsefly, Tabanus proximus

Int. J. Invert. Reprod.

The neural control of muscular activity in the hindgut of the cockroach Leucophaea maderae: prospects of its chemical mediation

Comp. Biochem. Physiol.

The oviduct musculature of the horsefly, Tabanus sulcifrons, and its response to 5-hydroxytryptamine and proctolin

Physiol. Ent.

Electrically excitable nerve cell bodies in the central ganglia of two insect species Periplaneta americana and Schistocerca gregaria

Comp. Biochem. Physiol.

Axon pathways of electrically excitable cell bodies in the insect nervous system

J. Physiol.

Motor neurones of the indirect flight muscles of Dysdercus fulvoniger

A. ent. Soc. Am.

Immunocytochemical identification of proctolin-like immunoreactivity in the terminal ganglion and hindgut of the cockroach Periplaneta americana

Cell Tiss. Res.

Octopaminergic modulation of neuromuscular transmission in the locust