Immunohistochemical demonstration of pro-γ-MSH-like substances in the pituitary gland of various vertebrate species

https://doi.org/10.1016/0016-6480(82)90156-3Get rights and content

Abstract

Using the peroxidase/antiperoxidase unlabeled antibody enzyme technique pituitaries of several vertebrate species: man, rat, pig, cat, duck, snake, toad, and fish immunostained with an antibody directed against a portion of the cryptic region of the corticotropin/lipotropin common precursor. In all of them, pars distalis corticotropes and pars intermedia melanotropes were positively stained. Specificity studies and controls performed ruled out the possibility of cross-reaction with other known pituitary hormones or peptides. The wide distribution of cross-reaction with the antibody among vertebrate species suggests that a sequence of the cryptic region may be responsible for a yet undiscovered biological effect.

References (24)

  • F.E. Estivariz et al.

    Purification and characterization of a γ-melanotropin precursor from frozen human pituitary glands

    Biochem. J

    (1980)
  • E. Follenius et al.

    Localization of anti-ACTH, anti-MSH, and anti-α-endorphin reactive sites in the fish pituitary

  • Cited by (7)

    View all citing articles on Scopus

    Sponsored by the Universidad Nacional de la Plata, Comisión de Investigaciones Científicas de la Provincia de Buenos Aires, and Consejo Nacional de Investigaciones Científicas y Técnicas de la República Argentina.

    2

    Members of the Investigator Career, Consejo Nacional de Investigaciones Científicas y Técnicas de la República Argentina. Dr. Iturriza is the Director of the Center and Professor and Chairman of Histology.

    View full text