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doi:10.1016/0014-5793(83)80606-1 
Copyright © 1983 Published by Elsevier B.V.
Synthesis in vitro of rat brown adipose tissue 32000 Mr protein
Karl B. Freemana, Sing-Ming Chiena, David Litchfielda and Hasmukh V. Patela
aDepartment of Biochemistry, McMaster University, 1200 Main Street West, Hamilton, Ontario L8N 3Z5, Canada
Received 24 May 1983;
revised 10 June 1983.
Available online 25 October 2001.
Abstract
The synthesis of the mitochondrial inner membrane 32 000 Mr protein from rat brown adipose tissue was examined. Polysomes from the tissue were translated in a reticulocyte lysate protein-synthesizing system and newly-synthesized protein isolated with a monospecific antibody against the 32 000 Mr protein. The newly-synthesized protein had the same relative molecular mass as the mature protein. It was taken up by mitochondria isolated from Chinese hamster ovary cells into a form resistant to trypsin.
Keywords: Brown adipose tissue; Mitochondria; 32 000 Mrprotein
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