Identification of multiple active growth factors in basement membrane matrigel suggests caution in interpretation of cellular activity related to extracellular matrix components

doi:10.1016/0014-4827(92)90397-Q

Experimental Cell Research

Volume 202, Issue 1, September 1992, Pages 1-8

https://doi.org/10.1016/0014-4827(92)90397-Q Get rights and content

Abstract

We have recently demonstrated the formation of interconnecting canalicular cell processes in bone cells upon contact with basement membrane components. Here we have determined whether growth factors in the reconstituted basement membrane (Matrigel) were active in influencing the cellular network formation. Various growth factors including transforming growth factor β (TGF-β), epidermal growth factor (EGF), insulin-like growth factor 1, bovine fibroblast growth factor (bFGF), and platelet-derived growth factor (PDGF) were identified in Matrigel. Exogenous TGF-β blocked the cellular network formation. Conversely, addition of TGF-β1 neutralizing antibodies to Matrigel stimulated the cellular network formation. bFGF, EGF, and PDGF all promoted cellular migration and organization on Matrigel. Addition of bFGF to MC3T3-E1 cells grown on Matrigel overcame the inhibitory effect of TGF-β. Some TGF-β remained bound to type IV collagen purified from the Engelbreth-Holm-Swarm tumor matrix. These data demonstrate that reconstituted basement membrane contains growth factors which influence cellular behavior, suggesting caution in the interpretation of experiments on cellular activity related to Matrigel, collagen type IV, and possibly other extracellular matrix components.

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¹: On leave from Department of Anatomy, School of Medicine, University of Zagreb, Salata 11, P.O. Box 286, 41000 Zagreb, Croatia.

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Identification of multiple active growth factors in basement membrane matrigel suggests caution in interpretation of cellular activity related to extracellular matrix components

Abstract

Cell

J. Biol. Chem

J. Immunol. Methods

Dev. Biol

J. Biol. Chem

Biochem. Biophys. Res. Commun

J. Biol. Chem

Biochem. Biophys. Res. Commun

Biochem. Biophys. Res. Commun

J. Biol. Chem

Biochem. Biophys. Res. Commun

Dev. Biol

J. Biol. Chem

Ann. N.Y. Acad. Sci

Eur. J. Biochem

Ann. Chir. Gynaecol

Jpn. J. Oral Biol

J. Cell. Biol

Biochemistry

Biochemistry