Type I collagen fibrils promote rapid vascular tube formation upon contact with the apical side of cultured endothelium

doi:10.1016/0014-4827(91)90194-Y

Experimental Cell Research

Volume 192, Issue 1, January 1991, Pages 319-323

https://doi.org/10.1016/0014-4827(91)90194-Y Get rights and content

Abstract

Extracellular matrix (ECM) components phenotypically modulate cultured endothelium. This paper examined the ability of ECM components to promote tube formation in vitro. When collagen type I was added to the culture medium of confluent neonatal foreskin or human umbilical vein endothelium at 10–100 μg/ml tube-like structures formed rapidly. Tube formation did not occur with the addition of other ECM components at similar concentrations. Electron microscopy revealed that the lumen of the tubes consisted of collagen fibrils, with the surrounding cells having typical endothelial junctional complexes. These findings demonstrate that when collagen fibrils make contact with the apical side of endothelium they act as a stimulus and provide a template for vascular tube formation.

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¹: Chris Jackson is the recipient of the Spurway Fellowship from the Arthritis Foundation of Australia and has support from the Wenkart Foundation.

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Short noteType I collagen fibrils promote rapid vascular tube formation upon contact with the apical side of cultured endothelium

Abstract

Cell

Cell

Blood

Biochim. Biophys. Acta

Science

Cancer Res

J. Cell Biol

Amer. J. Pathol

J. Cell Biol

J. Cell Biol

Lab. Invest

J. Cell Biol

Short note
Type I collagen fibrils promote rapid vascular tube formation upon contact with the apical side of cultured endothelium