Immortalized differentiated hepatocyte lines derived from transgenic mice harboring SV40 T-antigen genes☆
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Acquired resistance of Listeria monocytogenes in and escaped from liver parenchymal cells to gentamicin is caused by being coated with their plasma membrane
2014, Microbes and InfectionCitation Excerpt :Our results indicate that L. monocytogenes not only in LPC but also escaped from LPC show resistance to Gm and suggest that the acquired resistance of L. monocytogenes escaped from LPC to Gm is caused by being coated with the plasma membrane of LPC. HepSV40 derived from Simian virus 40 large T antigen transgenic mice [31] and AII derived from p53 knockout mice [32] were used in this experiment (Fig. S1). Both LPC lines were incubated in antibiotics-free RPMI 1640 (Nissui Pharmaceutical Co., Ltd., Tokyo, Japan) containing 10% fetal calf serum (Bio West, Nuaillé, France) (designated as complete medium (CM)).
Generation and characterization of islet cell tumor in pTet-on/pTRE-SV40Tag double-transgenic mice model
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2005, Biochemical and Biophysical Research CommunicationsThe effect of sequestration by nontarget tissues on anti-tumor efficacy of systemically applied, conditionally replicating adenovirus vectors
2003, Molecular TherapyCitation Excerpt :During amplification of Ad.IR vectors in 293 cells, the small “delta” genome that is created upon recombination is packaged; however, particles containing this delta genome band at a lighter density in CsCl gradients and can completely be removed from particles the contain full-length genomes [39,41]. 293 (Microbix, Toronto, Canada), HeLa (ATCC No. CCL-2), and HepSV40 [33] cells were grown in DMEM with 10% fetal bovine serum, 2 mM l-glutamine, 100 U/ml penicillin, and 100 μg/ml streptomycin. HepSV40 are normal mouse hepatocytes immortalized with SV40 large T antigen.
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2002, Mechanisms of development
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This work was supported by grants from the Deutsche Forschungsgemeinschaft, the Bundesminister für Forschung und Technologie (Bonn), and the Forschungsrat Rauchen und Gesundheit (Hamburg).