Elsevier

Experimental Cell Research

Volume 148, Issue 2, 15 October 1983, Pages 293-302
Experimental Cell Research

Relationship between histone phosphorylation and premature chromosome condensation

https://doi.org/10.1016/0014-4827(83)90153-2Get rights and content

Abstract

The relationship between histone phosphorylation and chromosome condensation was investigated by determining changes in phosphorylation levels of histones H1 and H3 following fusion between mitotic and interphase cells and subsequent premature chromosome condensation. We detected significant increases in the levels of phosphorylation of H1 and H3 from interphase chromatin in which a majority of nuclei had undergone premature chromosome condensation. In addition, we noted significant decreases in the phosphate content of the highly phosphorylated mitotic H1 and H3, presumably resulting from phosphatase activity contributed by the interphase component of mitotic/interphase fused cells. These observations further strengthen the correlation between histone phosphorylation and the changes in chromosome condensation associated with the initiation of mitosis. This study also suggests that maintenance of the mitotic chromosomes in a highly condensed state does not require the continued presence of histones in a highly phosphorylated form.

References (25)

  • LR Gurley et al.
  • DB Marks et al.

    J biol chem

    (1973)
  • LR Gurley et al.

    J biol chem

    (1975)
  • HR Matthews

    J theor biol

    (1980)
  • JR Paulson et al.

    J biol chem

    (1982)
  • PN Rao et al.
  • D Bootsma et al.

    Exp cell res

    (1964)
  • SK Hanks et al.

    Exp cell res

    (1982)
  • W Wray et al.

    Exp cell res

    (1970)
  • S Panyim et al.

    Arch biochem biophys

    (1969)
  • MM Bradford

    Anal biochem

    (1976)
  • PN Rao
  • Cited by (0)

    Address: Division of Cellular Biology, Department of Basic and Clinical Research, Scripps Clinic and Research Foundation, La Jolla, CA 92037. USA.

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